In silico PCR-RFLP
This script is an implementacion of in silico PCR amplification
and restriction digest tool programs.
For information about usage of in silico PCR
amplification tool check here.
For information about usage of restriction digest tool check here.
This script will compute amplicons yielded by PCR
amplification, and position of amplicons within genome and their length
will be shown. In a second step, the amplicons will be digested with
selected endonucleases, and length of resulting fragments will be
calculated. Results from this second step is shown in a table (next to
length of amplicons obtained in first step).
The data above and a simulation of bands pattern after
electrophoresis are shown in the response page.