Snake Charmer
This page has been created by
Christoph Grunau
to be used at insilico.ehu.eus.
Restriction enzyme digest of bisulfite treated DNA (
COBRA
):
differences between methylated/unmethylated sequences
Restriction Home
Tidy Up Sequences
(methylated)
ATGtAGAtcg ATGTcgATGA cgTGAtTGTA GAAAAttAGT GATttTAcgA cgTtTTA
Sequence 1 non-methylated
(will be automatically generated)
ATGTAGATtG ATGTtGATGA tGTGATTGTA GAAAATTAGT GATTTTAtGA tGTTTTA
Do not align
Align sequences (beta) and show best results
Minimum recognition size for each restriction enzyme
4
5
6
7
8
Type of restriction enzyme
All
Blunt ends
Overhang end
Commercial source
All
Amersham Pharmacia Biotech
Minotech Biotechnology
Stratagene
Fermentas AB
Qbiogene
American Allied Biochemical, Inc.
SibEnzyme Ltd.\n
Nippon Gene Co., Ltd.
Takara Shuzo Co. Ltd.
Roche Applied Science
New England Biolabs
Toyobo Biochemicals
Megabase Research Products
CHIMERx
Promega Corporation
Sigma Chemical Corporation
Bangalore Genei
MRC-Holland
EURx Ltd.
Only restriction enzymes with known bases (no N,R,Y...)
DNA sequences might be pasted as shown or just as a one line string (number, spaces and line feeds will be ignored)