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to be used at insilico.ehu.eus.
Restriction enzyme digest of bisulfite treated DNA (
differences between methylated/unmethylated sequences
Tidy Up Sequences
ATGtAGAtcg ATGTcgATGA cgTGAtTGTA GAAAAttAGT GATttTAcgA cgTtTTA
Sequence 1 non-methylated
(will be automatically generated)
ATGTAGATtG ATGTtGATGA tGTGATTGTA GAAAATTAGT GATTTTAtGA tGTTTTA
Do not align
Align sequences (beta) and show best results
Minimum recognition size for each restriction enzyme
Type of restriction enzyme
Amersham Pharmacia Biotech
American Allied Biochemical, Inc.
Nippon Gene Co., Ltd.
Takara Shuzo Co. Ltd.
Roche Applied Science
New England Biolabs
Megabase Research Products
Sigma Chemical Corporation
Only restriction enzymes with known bases (no N,R,Y...)
DNA sequences might be pasted as shown or just as a one line string (number, spaces and line feeds will be ignored)