Restriction enzyme digest of DNA
with commercially available restriction enzymes

Beta version: correct behaviour in being checked

Paste the sequence in the textbox as plain text, fasta or upload sequence.


  • Upload file:
    The file must be a text file with code in plain text or in fasta format (<3 MB)
  • No sequences available in the server


Display of Results: Table Tabulated text
Show sequence(s) on top
When two or more input sequences are searched:
Show only endonucleases showing different restriction patterns for searched sequences.
Settings: Default Change
Select specific endonucleases: Select

Version: 1.20080403 , based in REBASE version 711
This service recognizes 253 different cleavage patterns (from all 624 commercially available endonucleases).
Copy and paste PHP script is freely available at biophp.org
Information uploaded to the server is associated to active session.
Restriction Home.