Primer3: design of detection primers

GTGGCCGAGAATGAAGGGCTCTGCAAGACGGGCAACGCGTGGGTCGTCTGCATCGAGCCGGACGTCTGCAAGACGCCCAT CGGCGACGAGATGAAGCCGGTGCCGTACCAGATCCGCGCCTGCCTGGCCGCCGGCGTGGACGAGGCGACCACGGTGAGGC TCGGGACGTTCGACACCGCGACGATGGCGAGCCGGATCACCACCGTCCAGGGCGACGAGCCGGGGACGGGCGGCGGCGTC AAGAGCGGCGTGAACCTGGGGTACTGCCGGCCGGTCACGCACAGCACGACCGTCCACGCGGGAGGGGCCAACGTCACCTA TCACACATCGCTGTACGCGATGAACTGTGCGGGCCCGGACGGGCCGGAGAACACGTTCGGCAAGGCGGTGTTCATGGCCC ACGGGTTGGCACCTTATGCAGGGCCGCTCGTGGACCTCAACAAGATGCGCGAGGAGGCCGAGAAGAGCTGGTGGGAGAAG GCCTCGGACTGGGTCCACACCGGGCTCGATATCGTCGGCTTCATCCCGGGCCTCGGGGAGATCGCCGACGGGCTCAACGC GGCCATCTACCTCGCGGAAGGCGACTATCTGAACGCGGCCATCTCCGCGGCGGGGATGATCCCCTTCGGGGGCGCGGCGG CCACGGGGGGGAGGCTGGCCGCAAAGGCGGGCAAGGCCGTCGCGGAAGCGGTCGGCAAGGAGGGGGCGCAAGAGGCCGCG CAGCAGGTCGGGAAGCAGGGGGCGGAGCAGCTGGGGAAAGAGGGGGCGGAGAAGGCGGGGAAAGAGGGGGCTGAGAAGGC GGGGAAAGAGGGCGCCGAGAAGGCGGGGAAAGAGGGGGCTGAGAAGGCGGGGAAAGAGGGCGCCGAGAAGGCGGGGAAAG AGGGGGCTGAGAAGGCGGGGAAAGAGGGCGCCGAGAAGGCGGGGAAAGAGGGGGCTGAGAAGGCGGGGAAAGAGGGCGCC GAGAAGGCGGGGAAAGAGGGGGCTGAGAAGGCGGGGAAAGAGGGCGCCGAGAAGGCGGGGAAAGAGGGGGCTGAGAAGGC GGGGAAAGAGGGCGCCGAGAAGGCGGGGAAAGAGGGGGCTGAGAAGGCGGGGAAAGAGGGCGCCGAGAAGGCGGGGAAAG AGGGGGCTGAGAAGGCGGGGAAAGAGGGCGCCGAGAAGGCGGGGAAAGAGGGGGCTGAGAAGGCGGGGAAAGAGGGCGCC GAGAAGGCGGGGAAAGAGGGGGCTGAGAAGGCGGGGAAAGAGGGCGCCGAGAAGGCGGGGAAAGAGGGGGCTGAGAAGGC GGGGAAAGAGGGCGCCGAGAAGGCGGGCAAGAAGGGCGCTGACGACGCCGCCGGCGGAGGTGCGAAGAAGCCGAAGAAGA AGGGCGACGACGGGGTCGTGGTCAAGGGGACCCCCGGCAAGGGAAGCAAAGCGGGGGGCGGGAAACGGAAAGGGGGTAAG CACCGCCGGAAAGAATCGCCGAAGAAAAAACGTAAGAAGAAGAATAAATGCACGACGGAGGGACACCCCGTCGACGTGGC GACGGGCGAGGTCGTCGACGAGGCGACGGATATCTCGTTGCCCGGCGTCATTCCGCTCGTCATCGAACGCCAATATGCTT CTTCGCGCTGCGACGACCGAGACGCGGCGCTGGGACCGGGCTGGTCTCACAGCCTCGAGCAGTGGATCGAAGAGGGCGAA GAGACGATCGACCTGCACGATGACGAGGGTCGATGGATCTGCTTCGACAAAGTCGGCGCCGGCGAGGCATCATTCGAGCG CGGCGAGCGGCTCGAGCTCCGCGTCGGCCGCGACGGGAGCTATCGCGTTTACGACCCGGAAAGCCGCATCGCCCGCGTCT TCGCCGGGCCGGGGCGCAAGGCGCTGCTCCGCTCGATCGAGGACGCGAGCGGCAACGCGATCACGCTCGATTACCACGAG GGATTGCTCTCGCGCGTGGTCGACACGGCCGGGCGCGAGGTCCTCTTCACGTGGGACCGCGGCCGGCTCGCGCGGCTCGA TGTGCGCGTCGACGGCCGCGTCGAGCAATGGGTGGAGTACCGGTACTCGAAGGCCCGAAACCTCGCCTCCGTGGTCGATG CGCTCGGGAACGTCGAGGAGTACGAGTACGACGGCGCAGATCGGATGATCGTCGCCCAGCTCAAGAATGGCGCGCGCTTC CAGTACACCTACGACGACGAGAGCGGGCGGTGCGTGCGGACGTGGGGACCCGATGGCCTGCACGAGGTGGAGCTCTACGC CGACGGGCGGACGCAGACGACGAGGGCGGAGAGCGAGGAGCCGAAGATCTACACATGGGACGACGACGGACTCGTCACGC GCGAAGAGACGACGACGGGCGTCGTCTTGGCGGAGCGCGAGTACGACGAGGACGGCCACGTCGTCGAGGAGCGCAACGGC GCGGGAGACACGACGCGCTTCGAGCGCGATGCGCGCGGCAACCTCGTGCGCGTGGTGGATCCGGCGGGCAACGTGACGAC CTGGGCGTACGAGGGGGATCTTCCCGTCAAACGCACGGGCCCCGACGGGCTGGAGACGCTTTACACGCACGACGCGCGGG GAAGCCTTACCGAGATTCATTACCCGACGGGCGTCACCTACAAGCTAACGTACGACGACCGAGGGCGCCTGTGCTCGATC GAGGGGGCCGAGGGGCCCGTCGTGCGGTATGAGTATGACGCCCGGCACAACGTGATCGCCGAGACGGACGCGCGCGGAGC GCGGACCCTGTTCGAGTACGACACACTCGGGCGTCCCGTGTCGATGACGGACGCGTTCGGGAGGGGGACGCGGCTCACGT ACGACCGGCTGGGCAGGCCGATCGAGATCCAGCGTCCAGATGGATCCGTCATGCAGCAAGCCTACGACGCGCAGGGTAAT GTCGCACGTGTCACGGATGCCCTCGGGCAAGTGACGGCGATGGAGTATGCCGGCACGGGAGTGCTCGCGCATGTCACGCG GCCCGAAGGGCAAAAGTGGTCCTTCCGGTACACCTCGAAGGAGCGGCTCCGGGAGATCGAGAACCCGCGTGGAGAGGTGT ATGGGTTCACGTACGACGCCGCAGGTCGCGTCGTCGAGGAGCGGACATTCGATGGACGGGTGCTGAAGTACGACTATTCG AAGGCCGGCAACCTGTCGCGAATCGATTATCCGGACGGGAGCTTTCGAGCGTTCAACCACGACCGGATGGGTAATATCGT CTTCGAGCAGTCGACCGACGGAATCGCGACCTATCCGCGCGACTCGCTCGGGCGGCTGCTCGCCGCGGTCGTGGAAGAGG GGGAGCACAAGATTGTCACGATGTTCGAGCGAGACCGCCTCGGGCAGATCGTGACGGAGCGGCAGGGAGACAAGGCGCTG CGGTTCGAGTACGACGCGCGCGGGCGGCGGACCAAGCGGGTGCTGCCGAACGGCGCGACGACAAAGTACGTGTATGACGC GCTGGATGCGCTGGTCGCCGTGGAGCACGACGGCTACCGGATGGAGATCGAGCGCGACGTGCTCGGACGGGAGGTCTGGC GCGGTCGTCCGGATGAGCAGGTGTCGATCCGGAGCGGCTACGACACGATGGATCGACTGATCGAGCAGCGGGTATCGGCG TCGACTCCCGGGACGGAGATCACCAAGGATCTCGTCGAGCGGAAATGGAGCTACGACGCGCTCGGGCGCGTGACGCGGAT CGACGACGGTCGATGGGGCGCGACCACGTACCGATACGACCGGATCGATCAGCTCCTCGAAGCACGACGCGGCTCGCTCC GCGAGGTGTTCGCATACGACGTGGCGGGCTCGCTGCGAACGATGCTCGAAAGCCTAGAGGCACAGGCGGGCTCACCGGAG TGGGAGATCGCGCCGGGGGATCTGCTGCGGCATGCGGGCAAGGCCAAGTACACGTACGACAAGCGGGGACGCCGGATCGT CAAGCTCGACCTCGGAGCGCGCACGGAGGGCGCTGAAGCGGCGGCGACCGAGTACGCGTGGGACTGTCGGGATCGGCTGC GCGAGGTGCGGCGGCCGGACGGGACGCGGGTGCGCTTTTCCTACGACGCGTTCGGGCGGCGGGTTCGCAAGGACGTCGTA CTGGCGGGAGCCGAGACGGCAGATCGTACGGTGGAGTTCCTCTGGGACGGGAATGAGCTGGCAGCGGACCTGGAGCGGAC GCGCGGAGCACGGGTGTTTGTGCATGATCCGGGGACGTCCGCGCCGCTCCTGCAAGCGGAGCAGGGGCAGGTGTTCGCGG TGGTGAATGACCACCTCGGGATGCCGAAGGAGCTCCTGGGGCCGGACGGGCGGGTGGCCTGGTCGGCGGCGCATTCGGCG TGGGGGCGGGTGGTCGAGGTCTTCAGGGATCAGGCGGCGCAGCACGCGCGGCCGGTGGAGAGCCCGTTCCGGCTGCTGGG GCAGTACGCGGACGACGAGACCGGGTTGTGCTATGTGCGGTTCCGGTACTGGGACCCGGACGTCGGGAGGTGGTGCAGTC CAGATCCGCTCGGAGTCGCGGGAGGGGCGAAGCTGTTTGCGTTCGATGGCAGTCCAACCCTGCTGGTGGATCCGCTGGGG TTGAACACGCGACCGCCCAAGAGGCTGAAGAAAGAGGACCCGGGTCTTCAGGATTTCATTCAGAAGCTTCAGCAGGCAGG CGTGAACGTAACGAATTCGAACATCGAGATCATGGGCGCGAGCGGAGAAATCCTCGGAGAAGTAGACGTCATCACCGATA AGGTGCTTATCCAGTACAAGAATGGCGCTTCGAGCCCCAACGCGATCATCAAGCAGGTCCTTGAGAAGACCGAACCATAC GTTACACGGCCGGTAGTGGTGTTCGTCAACGATACAGGAAAATCAGGAGACAGGACCGTCAAGAAGGCTGGCTCAAAGAT CTGCGTCACGAACAACTTCGATCTGCTCGTGGAGGTTATCAAATAG

Length of primers: Minimum Optimum Maximun

Tm of primers: Minimum °C Optimum °C Maximun °C

Length of amplicon:
First option
Aditional default options: 150-250, 100-300, 301-400, 401-500, 501-600, 601-700, 701-850, 851-1000.

NOTE:
This form has been simplified from the original primer3 form .

Length of primers:	Minimum	Optimum	Maximun
Tm of primers:	Minimum °C	Optimum °C	Maximun °C
Length of amplicon: First option Aditional default options: 150-250, 100-300, 301-400, 401-500, 501-600, 601-700, 701-850, 851-1000.