Primer3: design of detection primers

ATGGACTCCGGTGCTGCATTCGAGACGCTCGTCGAGCTCGGCCGTGGCCGCGCGGCCCGACTCGGTGACGAGCTCGCGTA CAGCTTCCTTGTAGACGGCGAGTCCGAGCGGCTCGATGTCACGTTCGCGGAGATCGATCGACGGGCGCGCGCCGTCGGCG CCGCGCTCCAGGCGATCGCGGCCGCGGGCGAGCGCGCGGTGCTCCTGTACGCGCCCGGGCTCGACTACGTCGCCGCGTTC CTCGGCTGTCTCTACGCCGGCGTGGTGGCCGTGCCGGCGTACCCGCCGGATCCGGCGCGCCTCGGCCGGACGCTGCCCCG CCTCGAGGCGATCGCCGCCGACGCGGGCGCCCGCGTCGTGCTCACCACCGAGGCGATCGCGGCGATGGGCGACGCGCTGG CCGAGCAGGCCCCGGCGCTGCGCGCGGGGCGGTGGGTCAGCACCGACGCGCTCGCGCCCGGCGTCGAGGAGGCCTGGCGT CCGCCCGCGATCTCGCGCGGGACGCTCGCGCTCCTCCAGTACACCTCGGGCTCGACCGGGGCGCCCAAGGGGGTGATGCT CACGCACGGCAACCTGCTCGCGAACCTCGGCGCGATCGCGCGCGGGTTCCGGACCGGCCGCGCGTCGCGCGGGGTGATCT GGCTGCCGCCGTTCCACGACATGGGGCTGATCGGCGGCGTGCTCGAGCCGCTCTACGCGGCGTTCCCGGTCGCGCTGATG TCCCCGCTCGCGTTCCTCGAGCGCCCGATGCGGTGGCTGCGCGCCGTCGCGCGCTTCGGCGCCACGGTGAGCGGCGGGCC GAACTTCGCGTACGACCTCTGCGCGCGCAAGGCGACCGCGGCCGACGTGGCCGCGCTGGACCTCTCGACCTGGGACGTGG CGTTCACGGGCGCGGAGCCGGTGCGGCACGAGACGCTGGAGCGCTTCGCCGCGACGTTCGCGCCGTGCGGGTTCCGGCGC GAGGCCTTCTACCCCTGCTACGGGCTGGCCGAGGGGACGCTGATCGTGACGGGCGGCGAGCGCGGCGCCGGGCCCGCGCT GGTCACGGTCGATCGCGAGGCGCTCGCCGGCGGGCGCGCGGCGCCGCCTGCGCCGGGGCGCGCGGCGCAGACGCTCGTCG CGTGCGGCCGCGCCGTCGACGCCGGCGGCGAGGTGCGGATCGTCGATCCGGCCTCGACGCGGGATCGAGAGGACGGCGAG GTCGGCGAGATCTGGGTGCGCGGGCCGAGCGTGTCGCGCGGCTACTGGGGGCGCGCCGAGGCGACCGCCGAGGCGTTCGG CGCGCGGCGCGAAGGCGCGCCGGACGAGGGGCCGTACCTGCGCACGGGCGACCTCGGCTACCTGCGCGACGGGGCGCTCT TCGTGACGGGGCGGCTCAAGGACCTGATCCTCGTCCGCGGCCGGAACCTGTACCCCGACGACCTCGAGCGCACGGCCGAG GCGAGCCACCCGGCGCTGCGCCGCGGCTGCTGCGCCGCCTTCGGCGTCGAGGCCGAGGGCGAGGAGCGGCTCGTCCTCGT CCAGGAGGTCGACCCGCGCGGGGCGCCCGACCTCGCGGAGGTCGTCGCGGCGGTGCGCCGCGAGGTGCAGCTCCAGCACG ACGTGGCGGCGCACGCGGTGGTGCTGATCGCGCGGGGGACGATCCCGAAGACCTCGAGCGGCAAGATCCAGCGCGGCGCC TGCCGCGCGGCGTTCCTGGCCGGCGACCTCGAGGAGCTCGCGCGCGGGTCGCTGGACGCCGGCGCGCCGGAGGGGCACGC GATCGAGGCGCCGTCGACCGACCTGGAGCGGGAGGTCGCCGCGATCTGGCGCGAGGTGCTCGGGGCGCCGCGGCTGGGGG CGACCGACGGCTTCTTCGAGCTGGGCGGCGACTCGCTCGCCGCGATGCAGGTGATCGGGCGCCTCCGGGCCCGGCTCGGG CTCCGCGTCGAGCTGAGCGCGCTCTTCGAGGCGCCGACGGTCCGCGCGCTGTCGCGCCGGCTCGAGGCCGCCGCCCGGCC GGCGATGGCCCTCGGCGCGCTCGGCGGTGGCGAGGGCGCCGAGGGCCCGGGGCCGATCCCGCGCGCGCCCGACGCGGCGG ATCACCCGCTCTCCTACGAGCAGGAGCGCCTCTGGATCCTCGACCAGCTCCTCCCCGGGCTGACGGCCTACAACCTGGCC CCGGCGGCGCGGCTCCGCGGCCCGCTCGACCGGGAGGCCCTCGCGCGGGCGCTCGAGGGGCTGATCAGCCGCCACGCCGC GCTCCGCGCCACGTTCGCGGCGAGCCCGGACCCGGTGCAGCGCATCGGCGAGCGCCGCGACGTCCCGGTGCGCTTCGTGG ACGTGTCCGACCAGCCCGAGGGCGAGCGCGAGCGCGCGGCGCGGCTCGCCGCCCGCGCGGAGCTCACGACGCCGTTCGAC CTCGCCGCGGGCCCGCTGCTCCGGGCCCGCCTCGTGCGGCTCGGGCCGGAGGAGCACGTCCTCGTGCTCGGCGTGCACCA CATCGCGATCGACGGGTGGTCGTTCGCGCCCCTCCTCCGGGACCTGGGCGCCTTGTACGAGAGCTGTCGTCGCGGGGGCG CTCCGGCGCTCCCGCCGCTCGCGGCGCAGTACGTCGACTTCGCCCGCTGGCAGCGGCAGGCGCTCTCGGGCGCGCGCCTC GAGGGGCTCGTCGCGTGGTGGGCGCGGCGCCTCTGCGACCTGCCGACGGTCGAGCTGCCGACGGATCGGCCGCGCGGCGC CGTCCAGACGTTCGCCGGCGCCGAGCGGACGGCGGCGCTCCCCCGGCGCCTGCTCGGCGAGCTCGACGCGCTCTGCCAGC GCGCGGAGGTCACGCGGTTCATGGCGTGGCTCGCCGTGCTCGCCGTGGCGCTCCGGCGCGCCACCGGCCAGGACGATCTC GCGATCGGCACCGCGGTCGCGAACCGCCCGCGCCCCGAGCTCGAGGGGCTCGTCGGCATGTTCGTGAACACGCTCGTCCT GCGCGTGGACCTCGCCGGCGACCCGAGCTTCGCCGAGCTGCTCCGGCGCGCCCGGGCGACGGCGCTCGAGGCGTTCGAGC ACCAGGACGCGCCGTTCGAGAAGGTCGTCGAGGCGCTGCAGCCGAGGCGTGACATGAGCCGGTCGCCGCTCTTCCAGGTG ATGCTCGTCGTCCAGAGCGCGCCGATGCCCGCGATGGCGCTCGGCGGCCTCGAGGTCGAGCTCCTCGACCTCGGGGTGGA GACGACGCGCTTCGACCTGCGCTTCTCCGTGGAGGCGCGCGGCGGCGAGGAGGTCGTCTCGCTGCAGTACAACACCGACC TCTTCGACCCGCAGACGGCCGACGAGCTCCTCGAGTCGCTCGTGCAGATCGCGGAGCGGGCGGTCCGCGCGCCGGAGGCG AGGGTCGCGGCGCTCCTGCTCACGCCCGAGGAGCGCCGGCGCGCGCTCGTGACGTGGAACGCGACCGAGGCGCCGTACGA GGACCACCTCTGCCTGCACGAGCCGTTCTTCGCGCGGGCGAGCGAGCAGCCGGAGGCGGTCGCGGTCGTCGCTGGCGGCC GGCGGCTGACGTACGCGGAGCTGCGCCGGCGCGCGCTCGCCGTCTCGGCGCGCGTCCGGAGGGCCGGGCAGGGGCGCGGC CAGCTCGTGGCGATCGCGATGGACAAGGGCTGGGAGCAGGTCGCCGCCGCGCTCGGCGTGCTGCACGCCGGGGCGGCGTA CCTGCCGATCGATCCCGCGCTGCCGCGCGAGCGGCTCGCCCACCTGCTCGCGCACGGCGAGGCGCGCCTCGCGCTCACGC AGTCGCACGTCGACGCGCGGATCGACTGGCCCGCCGGGGTCGAGCGCGCGCTCGTGGACGAGCTGGACGAGCTGGGCGAG CTCGACGGCGATCTGCGCCTCCCTCAGCGCGCCGCGCCCGCGCCGGGCGACCTCGCGTACGTCATCTACACCTCCGGATC CACCGGCCAGCCGAAGGGGGTGATGATCGACCACCGCGGCGCGGTGAACACGATCCTCGACGTGAACCGCCGGTTCGGCG TCGGGCGCGACGACCGCGTGCTCGCGCTCTCGTCCCTGAGCTTCGATCTGTCGGTCTACGACCTCTTCGGCACGCTCGCG GCGGGCGGCGCCATCGTCCTGCCACCGCCGGGCCCCGGGCCGCAGCCCGACGCGTGGGCCGCGCTGGTGCGGGCCGAGCG CGTGACCGTCTGGAACTCGGTGCCCGCGCTGCTCCAGCTCGCGCTGGACGCCGCCCCCGCCGGCGCCAGCACGATGGCGC CGCCCGATCTCGCGTCGCTCCGCCTGGTGTTGCTGAGCGGCGACTGGATCGACCTGAAGCTCCCCGAGCGGATCCGGGCC GCGTCCCCGTCCGCGCGGATCGTCAGCCTGGGCGGCGCCACCGAGGCGTCGATCTGGTCGATCCTGCACCCGATCGACCG CGTCGATCCGCGCTGGCGCAGCGTCCCGTACGGCCGCCCCATGGCGAACCAGCGCGTCTACGTCCTCGACCAGGCGCTCG AGCCGTGTCCGATCGGCGTCCCGGGCGAGCTCTACATCGGCGGCGTCGGCGTCGCGCTCGGCTACTTCCGCGACGAGGCC CGGACCGCCGCGAGCTTCGTCACGCACCCGGCGACCGGGGAGCGGCTCTACCGCACCGGCGATCTCGCCCGGTACTTCCG CGACGGCGCCGTCGAGCTGCTCGGGCGGGTCGACTTCCAGGTGAAGGTGCGGGGCTACCGCGTCGAGCTCGGCGAGATCG AGGCGGCGCTGCTCCGGCACCCGGACGTGAAGGAGGCCGCGGTCGCCGTCAAGCAGGACCCCTCGGGCGACCGGCGCCTC GTCGCGTACGTCATCGGCGCCGGCGGCCTGGATCCGCAGGGGATCCTCGCGTCCGCGCGGCGGGCGCTCCCCGAGTACAT GGTCCCGAGCGCGTGCGTCCCGGTGGACGCCTTCCCGCTGAGCGCGAACGGCAAGGTCGACCGCGCGGCGCTCGCCGCGA TCGACCCGCGCGGCGGCCATGGCGAGGCGCCGCGGATCCCGCCGCGCACGCCGACCGAGCGCGCGATCGTCGGGGCGCTC AAGGAGGTGCTCCAGGTCGAGCAGATCGGCGTCAACGAGAACCTCTTCGAGATCGGGCTGACGTCGCTGCTCGCGGTGCG CGCGCAGCGGCTGCTCAAGGAGCGGCTGCGCGCCCCGGTCGCGGTGGCGGACCTGTTCCAGTCGCCGACGATCGAGGCGC TCGCCGCGCGCGTCGACGCGAGCGATCCGGCGCGCGGGTCCACGGACGCGGCCGAGCAGCGCGCGGACGCGCGCCGGGAC GCGGCGCGCCGCCGGCAGGCCGCGCGCGGGGATCGGTCGAACGGATGA

Length of primers: Minimum Optimum Maximun

Tm of primers: Minimum °C Optimum °C Maximun °C

Length of amplicon:
First option
Aditional default options: 150-250, 100-300, 301-400, 401-500, 501-600, 601-700, 701-850, 851-1000.

NOTE:
This form has been simplified from the original primer3 form .

Length of primers:	Minimum	Optimum	Maximun
Tm of primers:	Minimum °C	Optimum °C	Maximun °C
Length of amplicon: First option Aditional default options: 150-250, 100-300, 301-400, 401-500, 501-600, 601-700, 701-850, 851-1000.