Primer3: design of detection primers

Length of primers: Minimum Optimum Maximun
Tm of primers:   Minimum °C Optimum °C Maximun °C
Length of amplicon:
  First option
  Aditional default options: 150-250, 100-300, 301-400, 401-500, 501-600, 601-700, 701-850, 851-1000.

NOTE:
This form has been simplified from the original primer3 form .