Primer3: design of detection primers
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Length of primers:
Minimum
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Optimum
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Maximun
12
13
14
15
16
17
18
19
20
21
22
23
24
25
26
27
28
29
30
Tm of primers:
Minimum
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
°C
Optimum
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
°C
Maximun
35
36
37
38
39
40
41
42
43
44
45
46
47
48
49
50
51
52
53
54
55
56
57
58
59
60
61
62
63
64
65
66
67
68
69
70
71
72
°C
Length of amplicon
:
First option
150-250
100-300
301-400
401-500
501-600
601-700
701-850
851-1000
Aditional default options: 150-250, 100-300, 301-400, 401-500, 501-600, 601-700, 701-850, 851-1000.
NOTE
:
This form has been simplified from the
original
primer3 form .