DNA sequence >NC_023150, from 2581502 to 2581573 (72 bp); Rhodococcus pyridinivorans SB3094 GCCACGGAGCTGAAGCGACCGACAGTCACGGGGCTGAAGCGACCGACAGTCACGGGGCTGAAGCGACCGA CA Translate to protein Restriction digest BLAST Design primers with primer3