Sequence retrieval

Genome: Leisingera methylohalidivorans DSM 14336 strain MB2, DSM 14336
Start position: 281443
End position: 281640
Length: 198

DNA sequence
>NC_023135, from 281442 to 281639 (198 bp); Leisingera methylohalidivorans DSM 14336 strain MB2, DSM 14336 CTAGTTCATTTCCAGTTCCATTTCCAGTTCCAGGCGCAGCGCCGCTTCGCGCACTCTCAGCTGTTCCAGC TGCAGCAGGCCGCGTGACCGGTCGAAGGTGACCAGCGGATCCCCGGCCCGAACCTCCGAACCCTGCTGCA CAAGAACGGTCGTCACGCGGCCGCCATGTTGGCTCTGCAAGGTGCGCAGGCCCCCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 281443-281640	Sequence	METH_01525 - hypothetical protein (Protein is coded in complementary strand; from 281640 to 281443)