Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 8686462
End position: 8687949
Length: 1488

DNA sequence
>NC_022785, from 8686461 to 8687948 (1488 bp); Streptomyces rapamycinicus NRRL 5491 TCATGGACCGGCGGCAGCGTCGGGGAGGTCGGGTATCCAGGAGCCGTGGATGCCCGCCGGGACGCGGTGG GGCAGCTCCACGGTGGCCACGGGGGCGGCCGTGATGTCCCGGGCGTCCAGGACGATCAGCTCGGAGGCGG CACGGCGGAGATCGCTGACGATGGTCAGCAGATAGCCGTCGTCCTCGCTCCCTCCACCGTCCCCGGACGC CTCGCCCGGGTGTCCGTCAGTGCTCCCGGCTGCGGGCACGAACACGGCCTCCCCGGGCATCCGGCCCGGT CCGGCCGGCGCTGTCTGCCGGGCCCCGGTGGCCGCGTCGTACTTGACCAGTGCGTACTCCTCCAGTCCGC TCCCGGGGAAGGCGACGGCGTAGGCATAGCGGCTGGGCCGCCCCAGCACGCTGTCGTTGATCGTGGGGAA CTCGGTGACCAGGTCGTCCAGCGGCTCCTCCCACGCGCTGCCCGAGGCGGGGTCGAGGATCCAGCGGTGG TGCAGGGAGCCGACCAGCGGAACGGCGGCGTGGCCCGGGGCGCCGGTCCACCACTTCCAGGAGGTCTCCC AGGCGGTGCGGTCATAGCGCGGCCCCTCGATGACGACGCGGCCCGCCGGATCCTCATAGGCGTTGGCGAT GTGCAGCAGGGCGCCGGGCTCCACCTCGCACCAGGTGACTCGGGGCGGCCCGGTGCGCGGCATGATCCCG ATGCGCGGCCGGTAGCCGTCGGACCAGCGGTAGGGGATCCCGGACCGCTCGGCGGGGTCGAAGACGACCG GCAGATCCAGCCACACCACATGGCGCTCGGTCAGCCCGAAGTCGTGCATCAGCGAGGGCCCCGCGCCCTT TATGGTCTCCTCGCGCACGATATGGCCGTCGCGGGACGCCACGTAGTAGACGAGGTGGGGCGGTAACGGG GAGTACGAGAAGAAGTGCAGCTCCCCGGTGACCGGATCCTCCTTGGGGTGTGCCGTCATGGCCCGGTTCA GCTTTCCGCCGAAGTCATAGGCGCCGACGGTGGCCAGCTCGGGGGTCAACTCATAGGGGAGGTTGGCCTC CTGGAGGGCCAGGATGCGGCCCGCGTGCTCGATCACATGGGTGCCGGCGACGCTTGCGGCGAGATCCTCC GCGCCGTCCGCACCCCCGTCACCCGCGGCCGCGCCCTCGCCGCCCGCGGCCGCCGCCTTGGCGTCGTGGA GGGCCGGTGTGCGCACCCAGCGGTTGCGGTACCACTCCGCTCTTCCCTCGCACAGCCGGAGCCCGTGGAC CATGCCGCTGCCCTTGAACCAGTGCGAGGGCACCACTCCGGGCGGGGGATTGTGGCCGTTGCGCAGATAG CGGCCGGTCAGCTCGGGAGGCAGGGAGCCGTGCACGGTGAGGCCCCAGATGGTGGACTCCAGCCCCACCG GGGCGTAATGGCCGCTGAGATAGGGCGGCTTGGCGGGCTTGGCGGGCTTGGCGGGGTTGGCTGATGCGTT GAAGAACCGGTCGCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 8686462-8687949	Sequence	M271_35370 - hypothetical protein (Protein is coded in complementary strand; from 8687949 to 8686462)
ORF... 8687946-8689001	Sequence	M271_35375 - NADPH:quinone reductase (Protein is coded in complementary strand; from 8689001 to 8687946)