Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 8657350
End position: 8658813
Length: 1464

DNA sequence
>NC_022785, from 8657349 to 8658812 (1464 bp); Streptomyces rapamycinicus NRRL 5491 TCAGGGGCCGGTGCCCCTTCGCCGGCCGGGCACCCGGATGCCGTAGCGGGCCATCTTGCGGTAGACGGTG GCGCGGCCCAGGCCCATCTTCGCCGCCGCCTGGGCCACCGTCGTGCCTGGTTCGGTCAGGCAGCGCACGA TCTCGTCCCGTTCGAGGAGCTGCAGCCGGGTCAGATGGCCGCCCGAGCCGGTGAACACCTCGGCCGGGAG GTGATGGGTGTCGATCGCATCCGTCCTCGACGCCGCCTCGCGCACCACCCGGCGCAGCTGCCCCACGTTC TCGGGCCAGTCGTAGGACGTCAGCGCGCGGGCCGCGGCGGACGTGAACGTCACCGTGGGTCCGCGGTGCC ACCGTGCGAAGTGGCGGGCCAGCGGCAGGATGTCGTCGGGGCGGAAGCGCAGGGCGGGCACCTCGACCAC GGTGTCGACCAGCGGCCTCAGCACGTCCGGGATCGCGGAGTACCACTGGGCCGTCACGGCGAACGGCGGC GGACGCCCGGACCCCTCTCCGGCCACCCGGCGCGCCTCTTCGAACAGCCGGGCCAGTTCGGTGGCCGCCC ACGCCGGCAGCCGGTCCACACCGGAGACGACGACGCAGGTGCTGTCCTTGCCGAGTTCGGGGGCCCACAG CGCCAGCCACGCGGCCACGTCGCCGGGCGCGGGTGTGCGGGCGCTGAGCAGGCGTTCCCGCGCCCCGGTC TGCCGACGGGCGGTGGCGACCAGCGCGGCCTTCCCCGCTCCCGGCTCACCGACCACCGCGACGATCCGTC CGCGGGCCGAGGCGCTGTGCGCGGCGGCGACGGCGTCCGTCCAGCCGGGGGAGAGCGCCTGGTGAGCGTG GTCGTGCTGGTGGTACCGGTCGGCGTACATCCGGAACACCTCGCCCCGGGGGGCCGGTCGGACCCTGCGG CCGGTCCCCCGGGCCAGCATGAGCGCCGCCGTGTTCCCGGCGGCGGCTTGCGCAAGGGCCAGCAACAGCT TCGAGGACGAGTCGGACCACGTGGTGAGGTTGACGCTGCCGACGAGCCCACCGGTCCCCGGGTCGAGCAC CGGTGCCGCGGCACAGGTGTATCCCCGCAGGCCGGTGCAGTAGTGCTCGTCCGCTCTCACCAGTGACGGA GCCCGATCCGTCAGGGCCAGGCCGAGCCCGTTGGTGCCCACGTCGCGTTCGGCGAAGGAGAAACCGGGCG CCAGATGGACCCGGTCCAGGGACCGGCTGATCGAGGCGTCGTCGCACACCCGGCACAGCACCAAGCCGTC GCCGTCGGCGATCATCATGCCGACCGCCTCGTTGGCGAGCGTGTCCCGCAGGCCCCGCAGCACCTCATGA CCGCACTCGTAGAACAGGGAGGCCGTGTCCACCGAACCGGTGAACACCGGCCGCATCTCCTCCGGCGACA GACCGTAGCGCCGGCTGCGCTGCCACGATGCCAGCAGGCGCGGGGCCAGGCGGTGGCTGTCCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 8656364-8657395	Sequence	M271_35260 - LacI family transcriptional regulator
ORF.. 8657350-8658813	Sequence	M271_35265 - hypothetical protein (Protein is coded in complementary strand; from 8658813 to 8657350)