Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 8321983
End position: 8323785
Length: 1803

DNA sequence
>NC_022785, from 8321982 to 8323784 (1803 bp); Streptomyces rapamycinicus NRRL 5491 TCAAGGGGTCGACTGACCCGCCCAGTCCGTCTCCAGCGCCGCCACCAGCCGGGCGCCGCCGCCCGAGGGG TCCGACTGCGCCTGGTCCAGCCGCAGATGCGCGGTACGGCCCCGGAGGGTCAGCGTCATCAGCTGGTTGC CGAACCAGGGCCCACCCGTCTTGTGCCAGCTCAAACGGGGGTGCGGCACCCGCCCATGGCGCCGGAACGC CCGGCCCAGGGCGCGGCCCGCCGCGCTCCAGCCGAAGCGGAAGCCCAGCCGTATCGAGGCGTAGATGCTG TTGTGGACCGGGGAGCAGGTCAACTGGCGCACCTGGCTGCGGGGCGGCCCGGACGGCCACCGCGACCAGT CCGGGGCGGCCACATAGGCGTGGTGCACATCGCCGGAGAGCACGCTGATCGTGGCGGGCGCGCCCGGTGC GCGGCCCACCGAGGCGATGGTGTCGGTGAGCGCGTCGAAGGACTCGGGGAACGCGGCCCAGTGCTCCAGG TCGCCGCGCTGCCGCAGATCCTCGGCGATCCGCGCCCAGCGCCCACCGCGTCCGCCGCCGCACACCGAGG CGTTCCACGCCTCGACGTCATGCACGAAGTGCGGCAGCAGCCAGGGCAGCGAGGTGCCGAGCAGCAGATG GTCGTAGCCGCCCTCGTGGTGACCGCCTTGGCGACCGCCGTGGTGACCCTCTTCGTAGCCGCCGGGGGCC GCCCCGGGCCTCTCCGGGCTGGGCTCCTCCGGGCCGGGGAAGCCGCCCACGCCGTCCATGGCCTGCTCGC GCACCCAGGCGAGCTCCTTGTCGGTCAGCATCGCCCGCCGGCCCTCCTCGAGCACCCGCGCCGCGCGGGT GTCGACCATCAGCAGCCGGGTGCGGCCGAAGTCGCGGCGGTAGCTCCAGCGGGTGTACCCCGGATCGGCG TCGGCGCGGGCGGCGAACTCCCGCAGCGCCCCGGTGCCGTCCTCCGCCGCGCGCACCTCCGCGTAGAGCG GGTCGGCGGCCAGTTCGGCGGGGGAGAGGTTGCCCAGGTGCTGATGGACCCAGTAGGACATCAGCCCGCT CAGTATCCGCTCCCGCCACCACGGGGTGGCGCGCATCCGGCGCTGCCACGCCTCGCTGGTGTTCCAGTCG TCGATGACGTCGTGGTCGTCGAAGATCATGCAGCTGGGGACGGTGGAGAGCAGCCAGCGCACCTCGGGGT CCAGCCAGGACTCGTAGTAGAGCCGGGTGTACTCCTCGTAGTCCGCCACCTGCTCGCCCGGCGGCTCGCT CAGATCGCGGCGCTCGGCCAGCCAGCGGCGGGTCTCGGCGGACGTCTCGTCCGCGTACACCTGGTCGCCC AGCAGCACCAGCACATCGGGGCGCGGGGCCTCCGGGGCGCGGGCGAGCGCCTGCGCGAGGGTGTCCAGCG CGTCGGGCCCCACGGGGTCGTGCGAGGCGTCGGAGGGCGGTGCGGCCCACCGGCAGGAGCCGAAGGCCAC GCGCACGGGCGCGGGGTCCGTGGAGTCGGCGCCCGGGGCCGGGGCCGGGGTGCGGATGGTGCTGTCGGGG AACGAGGAGTCCGGCAGCGGCCAGACCTGCTCGCCGTCCAGCAGCACCCGGTAGGCGGTCTCGCTGCCCG GCCGCAGCCCGGTGACCGGGATCAGGGCGTAGTGGTGGTCCGCGATCCGCCAGGTGCGGGCCGTGCCGCC CGCGCCGTCGGCGCACCGCACCTCGGCCTCACAGGGCCGGTCGGCCTCGACCCAGACGGTCGCGCCGGTG CCGGTCTCCCAGTCGACATGGCGCAGCAGTGGTCCCAGGCGCAGCCCGGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 8321983-8323785	Sequence	M271_33830 - alkaline phosphatase (Protein is coded in complementary strand; from 8323785 to 8321983)