Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 8285590
End position: 8288835
Length: 3246

DNA sequence
>NC_022785, from 8285589 to 8288834 (3246 bp); Streptomyces rapamycinicus NRRL 5491 ATGCTGGGGCCACTCGAGGTTCGCACGGACGACGGCCGCTCGGCCGACGTGCCGGGGGCGCGGCTGCGCG GACTGCTGGTCGCCCTTGCGCTCAGGCCGGGACAGGTGGTTCCGAAGGCGTCGCTGGTCGACTGGATCTG GGGTGAGCAGCCGCCCGCCGATGCGGCGAATGCCCTGCAGCGCTTGGTCTCCCGGCTGCGGAAGGTGCTG CCGGGCGGGGCGATCGAGGGGCAGCCGGACGGCTATCGGCTGATGGTGGAGCCCGATGCCGTGGATGCCG TGCGGTTCGAACGGCTCGTCGGCGCGGGTCAGGCCCGCGCCGGGGACGGGGCCCGGCGGGTGCGGCTGTT GCGCGAGGGCCTCGGCCTGTGGCGCGGTGCGGCCATGCAGGACGTCGGGCTGCGGGACAGCGCCGCGTTC GACGCCGCGGTCGTACGGCTCGAAGGGCTGCGGCTGACCGCCACGGAGGAGCGGGTCGAGGCGGAAGTCA CCCTCGGGCGGGGTGCGGACCTGGTGGCGGAGCTGACCGACCTGGTGGCCGCGCACCCGGTGCGGGAACG GCTCGTCGGCGCGCTGATGCGGGCCCTCGTCGCGGCCGGTCGGGACAGCGAGGCGCTGCAGGTGTACCAG CGCGCGCGGGAGGCGCTGGCCGACGCGCTGGGCGTCGATCCCTCGCCGGAGCTGGCCGCGTTGCACATCG CGCTGTTGCGGGGCGAGCTGGGGCGGGGCGAGCCGGGACGGGGCGAGCCGGGGCGGGGGGAAGCGAACCG TAAGAGCAACCTGCGCGCCGAGCTGACCAGTTTCGTCGGCAGGGACGCCGATGTCGCCGCGGTCCGCGGG CTGATCGCCGAGCACCGCCTCACCACCGTGATCGGGCCGGGCGGCGCGGGAAAGACCCGGCTGGCCACGG AGACCGCGCGCACGCTGCTCGGCGAACTGCCGGACGGCGCCTGGCTGGTGGAGCTCGCCGCCGTTGGCGC GGACGGCGATGTGGCGCAGCCCGCGCTCGCCGGGCTCGGCCTCCGGGACGCCCTGCTCGGCGCGGCCCCG AACGCGGAGCTGACCGACCGGCTCATCGCCGCGGTCCGCGAACGGGAGGCGCTGCTGATCCTGGACAACT GCGAGCATGTGATCGAGTCCGCGGCGGTATTCGCCCACCGGGTGCTCGGGGAGTGCCGACGGCTGCGGAT CCTGGCGACGAGCCGGGAACCGCTCGGCATCACCGGGGAGGCGCTGTGGCCGGTCGAGCCGCTGGCCCTG CCGGAGGGGGACGCGGGCCCGGGCGAGATCGGCTCCTCACCCGCCGTCCAGCTGCTGCGGGACCGGGCCG GGGCGGTACGCCGTGGCCTCGCGGCCGACGCCCACACGCTGGCGACGATGGTGCGCGTCTGCCGGGCGCT GGACGGGATGCCGCTGGCGATCGAACTGGCCGCGGCCCGGTTGCGGACCATGTCCATCGACCAGCTCGCC CACCGGCTCGACGACCGGTTCCGCCTGCTGACCAGCGGCAGCCGCACCGCGTTGCCGCGGCACAAAACGC TGCGCGCGGTCGTCGACTGGAGCTGGGAGCTGCTCACCGAGGCCGAACGGATGGTGTTGCGGAGACTCTC GGTGTTCTCGGGCGGGGCGAGCCTGGAGGCCGCAGAACGGGTCTGCGCGGGCGGCGGAGTCGAGCAGGAG CGGGTGCTGGAGCTGCTCACCGCGCTGACCGAGAAGTCGCTGCTGGTCGCCGAGGGCGGTGGCGCCCCGC GCTACCGCATGATCGGCACGATCAGGGAGTACGCCGGACACCGGCTCGCGGAGGCGGGGGAGTCGGACCT GGCGCGCCAGGCGCATCTCGCCCACTTCACCGAGCTCACCGAGACCGCGGAGCCGTGGCTGCGCCGCGCC GAGCAGGTGGAGTGGCTGGCCGTGCTCGAGGCCGAGCACGACAACATCGGTGCGGCGATGCGCGGCGCGC TCGCGGCGGGCGAGGCGCGGGCGGCGATGCGGCTCGCGGCGGGCGCGGGCTGGTACTGGTGGCTCGGCGG GCGCAGGACCGAGGGCCTGGAACTGATCACCGCGGCCACCAGGACGCCAGGGGAAGTACCCGATGACGTA CGGGCCTTGGTGTACGCGCTGATCGTGCACTTCGTGACCTCCGGGCGGAGCGATGAGCGCCAGGCCGCGG AGTGGATCCACGAGGCGCACCGGCTCAGCGCGCACGGCCACTGCCACCCGGGGGTGAAGTTCATCGCGGT CCTGGAACGCATGCTGCACACGCCCGAAGCGGCCCTGACCGCGTTCGCACCGCTGCTGGACGACGATGAC CCCTGGGTGCGCGCGCTGGCCCGGTGGCATATGGGCAAGATGCGGATCATGTTCGGGCAGGCCGGGCAGG CCGGGCGGGACGGGCAGGCCGGGCGGGACGGGCAGGACGGGGACGGGCAGGACGGTCGGGGTGGCCAGGA CGGGCAGGACGGCCCGACCGCTGAGGCCCACCTGGAGGCGGCGCTCGCCGAGTTCCGGGCGCTCGGCGAG CGGTACGGGATCTCGCTCGCCCTGACCGAGCTGGCCAACCTGCTCGCCGTACGCGGCGACTTCGCCGGTG CGTGCGAACGCTACGAGCAGGCGATCGCGGTCGTCAGCGAGGTCGGTGCCACCGAGGACGTCATCCGGAT GCGGTCGCGGCAGGCGCAGCTGTACTGGCTGCTGGGCGACAAGGACGCCGGCGCGGCCGCCATGGCCGAG GCGCGGCGGTGCGCGGAGCGGGTCACCTGGCCGGACGCGCTGGCCGAACTGGCCTTCTCGCGGGCGGAAC TCGCCCGGTGGGACGGCGACGCCGAGGAGGCGCACCGCCAACTCGGCCTCGCGACGGCCATGCTGGGCGA CGAGGCGGAGCGGGCGGACATCCGCGCGGTGACACACGATCTGCTCGGCTACCTCGCCGATGACCTCGGC GAGGTCCGGGCGCACCGCGCGGCGGCCTGCGAGGCGGCGTCCGAGGTGGGACACGCGCTGCTGATCGCGC ATGTGCTGGTCGGGGTCGCGGACCTGGCACTGCGCCGCGACGAGCACGAGCAGGCCGCGCGGCTGCTCGC GGCGAGCGCGGTCGTACGCGGGCTGCGGGACCGCGCCCACCCGGACCTGGCCCGGATCGAGCGGGCCGCG CGGTGCCGCCTCGGCGAGGCGAGGTTCGCCGAGGCGACTCGGGACGGGACGCGCACCAGCTGGTCCCGGC TGGTCGAGGTCACGCTCGCTTCCTGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 8285590-8288835	Sequence	M271_33635 - hypothetical protein
ORF... 8288819-8289583	Sequence	M271_33640 - ABC transporter permease (Protein is coded in complementary strand; from 8289583 to 8288819)