Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 8147711
End position: 8149540
Length: 1830

DNA sequence
>NC_022785, from 8147710 to 8149539 (1830 bp); Streptomyces rapamycinicus NRRL 5491 TCAGGACCCTCCATCGTTGGTCAACAGGTGGAGCAAGGGGGTGGCGGGGAGCGGTTCGTCGCTCTCCCAG GTACGGGCGAGCGCGCCGACGGTGCCGAGATCGCCGAGAAGGATGCCCCCGAGCAGACGGTCACCGCGGA CCACGACTTTGCGGTACGTGTTGCGAGTGGCGTCGGTGAGGTGAATGACGTCGTCGCCGGGGGCGGGGGT GGGGTTGCCGAAGGCAGCGAGGTCGAGGGGCCCGGGGGCGGAATGCCCTTCGGCCGCTGTGGTGAGGGTG AGGCGGGTGAGCGTACGGGTGCCGTGGTAACGGGGCGGGGTTTCGTTGCCGGGTGCGGCCCGGTGGCCGG GTTGTGCCCACTGGTTCCGCGCGGCGTGGTCTGGGAGAGCGGTGTCCGTGTCGCGGGGCGGGGTTTCGTT GCCGGGTGCGGCCCGGTGGCCGGGTTGTGCCCACCCGTTCCTCCCCCAGCTACCGCTGGGAGGTGCCCCC TCCGCGGAGCGATTGCCCACAACCTGGTCCCCAGGTGCGGGGTCGGGGGCGGAGCCCCGCTCGGCCCCAG GGGCGGAGCCCTGGTCGGGTCCTGGGGCGGAGCGCTGGTCGGGGTTCGGGGCGGAGCCCCTGCGGGGGGT GGGGGCAGAGCCCCGGCTGGGTCCGAGGGCGGAGCCCTGGTGGGGGTCTAGGGGGCGGAGCCCCATAGGT TCGGGGAGGGGCGGGGTGGGGGAGAGCAGGCTCGCCAGCACGTCCGCCTGTTCCTGCGCGGCGCCTGCCA ACCCGTACACGACCCCCGCGTGCTCCGCGCAGTCACCGATCGCGAACACCCGCGGATCACTCGTCCGCAG CTCGTCGTCGATCACGACCCCCCGCGCCACCTCCAGCCCAGCGGCCACCGCCAGCCCGACCCGTGGCCGG ACCCCGCACGCCAGCACGACCAGGTCCGCCTCCAGGGCGTATCCGTCGGCCAGTTCGACGCCGCTCACCG CGCGCCCGCCGCTCACCGCGCGCCCGCCGCTCACCGCGCGCCCGCCGTCCACCGCGCGCCCGCCGTCCAC CGCGCGCCCGCCGTCCACCGCGTCCCCCGTGCGCGTGCGCAGGCCGCGGACGCGGCACTCGGTGTGGACC TCCACGCCCAGCGCCGCCATATGCCGCCGCAGGACCCCTGACGCGCCCGGGTCCAGCTGGCGCTCCATCA GGTGCTCGCCCTGCTGCGCCAGGACCACCTGGGCGCCGCGCTGGGCCAGCGCACGCGCGGCGGAGACGCC CAGGAGCCCGCCGCCGATGACGACGGCCCGCGCCCCGGGCCGTACGGCCGCGCCGAGCGCCAGGCAGTCG TCCATGGTGCGGAAGGCGTGGACGCCGTCCGGAAGCTCGTGGCCGTCCGGTTCGAACAGGCCGCGCAGCG GCGGCAGGACCGGGTTGGAGCCGGTGGCCAGCACCAGCGCGTCGTACGGCACGCGGTGGCCGTCGTCGCA CACCACCTCCGCCGTGGGACGGTCGATGCGCACGACCCGCACCCCGCTGAGCCGCCGTATCCCGGCGGCG GGGGCGGGGAGCGCGATGACCTCCGGCGCGTACCGCCCGGCGAGCACCTCGGCGAGCAGCACCCGGTTGT ACGCGGGGTGCGGCTCCTCGCCGATGATCGTGACCTCGACAGCCACCCTGCCGGCGCCGGTGTCGTCGGT GCCGGTGCCGGTGCCGGCTGCCGAGCCCGCACCGGGACCGGCGACCGCGCCGGCACTCGCGCCCGCGCCC CGCGCAACAAGCTGCTGTGCCAGCCTCGTGCCCGCCATGCCGCCGCCGACGATCACCACCCGGCGGCGGC TCGGGCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 8147711-8149540	Sequence	M271_32980 - hypothetical protein (Protein is coded in complementary strand; from 8149540 to 8147711)