Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 7586660
End position: 7588852
Length: 2193

DNA sequence
>NC_022785, from 7586659 to 7588851 (2193 bp); Streptomyces rapamycinicus NRRL 5491 TCACTCACGCTCCTCGCTCTGCCGCGGCTCGTGCGGGGTGACGACCGCCAGCAGCTGATCCATGGCGACC GTGCTGCCGGGGGTCACCTCGAGCTCGGTGACGGTGCCGTCGTGCGGCGCGGAGATGAGGTGCTCCATCT TCATCGCCTCGACCACCAGCAGGCCCTGACCCGCGGTGACCTCGTCGCCGACGGCCGCCTTCACGACGGT GACGGTGCCGGGCATGGGCGCGGTCAGCTCCCCGGCGTGCGCCGCGGCGGCCCCGCCGCGCAGCGCCTCC TCCACGGGGTCGTGCCCGACCACGCGCCAGGCGTCCCCGTCCCGCCCCAGCCAGTGGCTCGCGCCGTGCG CGGCATGCCGGAAGCGATGGGTGACCCCGTCGAGGTCCAGCAGCACGGAGCCCTCTTCGAGGGCAATGAG GCGTGCCCGTAGGGTGGCGCCCGCGTCGGCACCGGCGGCGTGGGCCGCGTCGTCGGGGTGCTCGATGCGG AGTTCCGCGTCGTGGGCGCGGCCGCGTACGCGGACCGTCACCGGGGGGTGGCCCGCTACGCGGAGGCGGT GGACCGTCCAGGCGGGGGCGCCGCCTAGGCGCCAGCCGGTGGGCACCGAGAACGGGTCGACCCATACGCC CGCGGCCGGGCCGCCGAGGCCGGAGCCGCCGGGCTCCGGGCGCCCGCCGGATCCATCGCCACGCGGAGTG GCGGGCTCCAGGGCCGCCTGGCGCAGCAGGGCCGCCGTCGCGTAGACCTCTTCCGGTATGGCCGCCGGGA TCAGCTGGGCCGCCGCGCGGTCGACCAGGCCCGTGTCGAGCTCGCCCGAGGCGACCGCGGGGTGGGCGGC CAGACGGCGCAGGAAGCCCGTGTTGGTGTCCACGCCGAGGACGGTCAGGGAGCCGAGGGCGGCGCGCAGC CGGCGCAGGGCGGTGGGGCGGTCGGGGCCATGGGCGATGACCTTGGCGAGCATCGGGTCGTAGACCGTGC CGACCTCGGTGCCCCGGCTCAGCCCGGAGTCGACCCGCACCCCCTCGCCCTCCGGCTCGTCCAGCGCCAG TACGGTCCCGGCCGAGGGCAGGAAGTCCACCCGGCCGTCGGCCGCCGCGCGGGCGGTCTCGGCGCAGATA CGGGCCTCCACGGCGTGGCCCGCGGAGGCGATGCCGTCCTGCCCGAAGGGCAGCGGCTCGCCCGCGGCGA CGCGCAGCTGCCACTCCACCAGGTCAAGACCGGGCCGCCCGCCGACCGACACCGTCAGCTCGGTGACCGG ATGCTCCACCTGGAGGCGGGTGTTCATCTCCATGAAGCAGTACGCGGACGGATCGTCGCCCGGCACGATG AACTCGACCGTGCCCGCGCCCACATAGCCGCACGACCGGGCGGCGGCCACCGCGGCCTCGCCCATCCGCG CCCGGGTGGCCTCGTCCAGCAGCGGGCTGGGCGCCTCCTCGATGATCTTCTGATGGCGGCGCTGCAGCGA GCACTCGCGCTCCCCGAGGTGGACCACGTGCCCCTGGCCGTCCGCCAGCACCTGGATCTCGATGTGCCGG GGCCGGTCGATCCAGCGCTCCACCAGCAGCGTGTCATCGCCGAAGGCGCCGCGCGCCTCCCGCCGGGCGG CGGCGATCTCGTCGCCGAGCAGCGCCTCGTCGCGGACCAGCCGCATGCCCTTGCCGCCGCCGCCCGCCGA GGGCTTCAGCAGCACCGGCATCCCGATCTCGCGGGCGGCGGCGGTGAGCTGCTCGTCCGTCAGCCCGCTG CCCGAGGAGCCCGGCACCACCGGCACCCCGGCCGCGCGCACGGTCTCCTTGGCGCGGATCTTGTCGCCCA TCAGCTCGATGGCCCCGGCGGGCGGGCCGATGAAGGCGAGCCCGGCGTCGGCGCAGGCGCGGGCGAACGC CCCGTTCTCCGCGAGGAATCCATAGCCGGGGTGGACAGCCTGGGCGCCGGTGCGCGCGGCGGCCTCCAGC AGCCGCTCGATGGACAGATAGCTCTCCGCGGCGGGGGCGGGGCCGATCCGCACCGCCGTGTCCGCCTCGC GCACATGGCGGGCGTCCGCGTCGGCGTCGCTGTAGACGGCGACCGAGCGCACTCCGAGCCGCCGCAGGGT GCGGATGACCCGTACGGCGATCTCACCGCGGTTGGCCACCAGGACGGTGTCGAACATCGCGGGCGACGGG CCGGTCGTCTTCGTACCGCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 7585728-7586660	Sequence	M271_31145 - hydroxymethylglutaryl-CoA lyase (Protein is coded in complementary strand; from 7586660 to 7585728)
ORF.. 7586660-7588852	Sequence	M271_31150 - biotin carboxyl carrier protein (Protein is coded in complementary strand; from 7588852 to 7586660)