Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 7534486
End position: 7536201
Length: 1716

DNA sequence
>NC_022785, from 7534485 to 7536200 (1716 bp); Streptomyces rapamycinicus NRRL 5491 TCACAGATAGTCCTCCTCGGTCATCGGGTCGGGCTGGGGGCCCTGGACCGGGGCGGCGGCGTCCGGCGGG CCGCCGTGGGTCCATGGGGTGCTATCTGCGTCGGGCAGCTCGGGGCCGCCGCGCGGGGCGTGCTCGAACA GCGTCCAGCACACCCGGTCGCCCTTCTCGGGCACCGTGCCCGGCTCGGGCTGCTTGCCCCGGCCCATTCC GCCGGTCAGCCGCACCACCAGCGCGCGGCCCTGGCCGTCCGCGTCGTCCGGGGCGTCCGCGTCGCCTGGG CCGTCCGCGTCGCCCTGGCCCTTCGCGTCGCCCTGGCCCTCCACGGCAGCGGCTCCGTCGGCCCCGTCGG CCCCGTCGGCACCATCCACGTTCGCGAGACCCGCGAACTCCCCGCTCTGTGCCCTGCCGTCGGCCGTGAC CCGGTGCAGCTTGACCCGCTCGGCCAGGTGCGGACGGTCCTCGGTGCGGATCGTGACCAGCGGGCGCGGG CGCGGCACCTTCGCGTCCGACCAGGCCATCTCCACATCGATCACCTCGCCCGCGAACGCCTCACCGGCCA GCCGCCGCCCCGCCATGACCAGCGGATCGTCCAGTGCCTCATGGGCGTCCAGCTGGGCCTGCGCGGTCTC GCGCGCGGCGAGCTTCTGCGCCGCCGTCACCGCGTCGTCCAGCTTGGGCTGCGGCGGCTCCCCGGCCCGG ACCCGGTCCCGGTGGCCGGTGTAGGACCAGCGGTCCCGCTTCCAGCGGTCGGCCACCCGCGCGCCCTCCG GGAGGGCGCGCAGCAGATCCACTCCGCGCCACAGCGCGTCCCAGGTGGGCCGCAGCTGTGACTCGATCAG CCCGCGCAGCTCCGCCTCGGCGAGCCGCAGCCGCGCCGCGCCCCCGGCCACGCCCTGTGACGCCTCGGCA CGGGCCGCGTCGTAGCGGGACATCGCCGGGGCCAGCAGCTTGTTGTCGAAGGCGGGGTCGGTGGCCGGGC CCGCGGGCGGGCAGCGCAGCCGACTGTCCCCGTCCCGCTCCGACTCCGCCCGCAGCGCCGCTTCCGCGCC CGGTACGCCCTCCGGCGGGTCGATCCAGGCCAGCAGCGAGCCCAGGTGCTGATCCTCCAGGTTGCTCTGC CCGGTCGCCCAGTGACGGCTCAGCAGCTCGGTGAGCGACAGCAGCAGACAGGAGCCGGGCACTCGCGCCC GCTCCCCGTAGTGGGTCAGCCACCGCCCCAGCAGCGGTACCCGCGCGGGTGCCGGGAAGGGCGTCTCCGG GTCCTGCTCGGCGGTCCGCCGGAACCGCATAGACCGGCCCAGCAGCCGGACGAACGCCACGCCCGCGGCG CTGGGCACCAGCAGCTGCGGCGCGTCCGCGCAGAGCTCGACCTGGACCGGCACCTTCTTACCGGTCTCCG GATCGGTCTCCTTGCGCTCCTCCAGCTCGACCTCGTCCCCGTACGAGTCCAGATACGGCTGGATCTCCTC GGCGAGCTCGGCGAGGAACGCGAACCTCAGGTCGCGGTCGCGTGGCTGGGCCACGATCAGCAGCCGCGGC GCGTCCCGGTCGGTGCCCACCAGTGCCCCGAGCGGCGCCCCCGCCTCACCCGCGGTGGTGAGCGGGACGA ACGCCAGCGGGCGCTCCGACAGATGGCGGTGGCGCACGGTGGTCAGGGGCTGGGCCCGCCCCTCCCGGAC CGCCTCCATCCGGGCCAGGGTGGTGATCAGCGACAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 7533104-7534489	Sequence	M271_30880 - helicase (Protein is coded in complementary strand; from 7534489 to 7533104)
ORF.. 7534486-7536201	Sequence	M271_30885 - membrane protein (Protein is coded in complementary strand; from 7536201 to 7534486)
ORF... 7536192-7537469	Sequence	M271_30890 - hypothetical protein (Protein is coded in complementary strand; from 7537469 to 7536192)