Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 7301086
End position: 7303281
Length: 2196

DNA sequence
>NC_022785, from 7301085 to 7303280 (2196 bp); Streptomyces rapamycinicus NRRL 5491 CTACCGGGTGATGGACCAGCGTCCGGCGCCGGGCGGCGAGGCCGTGTGGAGGTCGCGCAGCCGGACGACC GCCTCTCCCTTCTCGGCGAGCGCGTCGACGGCGTCGGTGAAGCGCTGGATGGACAGTGGTGTGGCGGGGC CGAGGAGAAACTCCTTCACCTCGCGGCGGGCGGTGCGCAGCTCGTCGAGGGAGGGGTCGAAGAGCGGGCG CAGGAGGTTCTGCATTCCTGATGCCCCGGGACCGAGCGGATAGGCCGCCCTGGCCGTGACGTGTCGCCGC CGGAACTCCTCCTCGCCGAGCCCTTCCGGGACGGTGAGGGCGTACGGCTTGAGGGTGGCGAGGAAGTCGC CGACCACGTCGGAGGGGTCACCGATCAGCAGATCCGCCTGGTCGAAGCAGGAGTGGAGGGTGGGGGCGTC GCCCTCGATCACCTGGTGGAGCCAGGGCGGTGAGGCCGCCCAGTGCGCCTGGTGCCACTCCCGGCGCAGC CCCTCGGCCTCGGTGAGCAGAGCCGGATCGGGTGCGGCGCGGTCTCTGGTGAGCATGGCCTCGTCGGCGT CCGCCCGGCGCAGCCGGGCGTCGAGCGCGGCCATGCGCTTGGTCAACTCGGCGCGGAGGCGGGCGGCTTC GCCGCGGCGGGCCTCGGTGCGCGGGGTGTCGGAGTGCGGGTCGTCGGCATCCGGGGCCCGGGCGTCGTCG GGCTCTTCGCCGAAGGCGTCCGGGGCTACGGCGTCCCCGCCGAGGGCTTCGGCGGCGAGGGCGTCCGCGC TGAGGGCGTCGGCGGCGAAGGTGGCCCTGCCGAGGGCGTTCGCGGCGAGGGCCTCCGCTGCGCGCCGCTC GTTCGCGGCCTGGATCAGGGCGGCGATACGGCGGTGGGCGGCGTGGTCCTCGGGGGAGCGGTGGCCGGTG GGCGGATGCGGCCGGTAGAGCACCCGGACCGGGCGTTCGCTCTCCAGCAGGGCCCGTACGATCGGCTCAC CCGCCGTGAGGTCACGGGTGGGCGCGTACAGCACGGTGGGCAGGGGCGTGACCGTGGGCGTCGGCGCGGG GGCATGACCGGTTCCCGTGACGCGCAGGCCGTCGAGCTGGGGGCGGCCGATCTCCACGATGTCGGCCTCC TCGACACCGACCGCGGCCCGCGCATAGCGGCGGCGCCCCGCCTCGCCCGCGACCCACACCTGGTCGTAGA CCTTGCTGTACGGATCGACGGCGGCGGTCTCGTCGCCGTCGCCGGGGGCCAGGAAGACGTGCTGCATGGT GGGGATCCGCAGGAGGTGGATGTTCCGGCCGGTGTTGGCGGGGTAGAGCGCCACCCGCACCCCGCTCAGG TCCAGCTGCGCCAGATGGGCGGCGCTCGGCACGCACAGCACCGGCAGCGGGGTCGGCCCCAGGGCCTCCA GCAGTTCCCGTTCGCGCAGGATGACCAGGGACGGGGTGGGCAGCGCGGCCACCGGCCCCAGCCATCGGGT GACCTGGTCATCCCGGTCGCGCGGGCCGGAGAAGTACAGCACCGTGCGGGGGCGGTGACGGATCAGCCAG GCGTCCACCTCGGCCAGCACCTCGGCGGCGCCGGGCAGCCGGCGGGCGGCCCGGACCAGGTACGGAACGA GCGCCACCACCGTGACCAGGGAGGAACCGGCGGAGAGCGTGAGCGCCGCCACCACCCAGCGGGCGTCACC GGTCGTCAGCGACGCGGTGAGCCCGAGCAGGGCGGACAGCTCGTAGACCAGGATCCGCTGACCGGGCCAT TCGGTGAGTCTGCGGGGCGGCCGGTCGCTGATGTCCAGTCGGCGCAGATCGATGTTGCGGGCCGCGACCG CGAGGGCGCGGCGCCGCCGGATGAGGGTGGTGAGCGCGGCGTGCAGGGCCTGCCCGGCGAGCAGACACAG CACCAGCGTCATGGCCGCGGCCAGTCCGATGGGCCCGGTGACATGGAGTCGGCCGAGCAGCAGGAGCAGC AGCACACAGCGGAACTCGAAGCGTGACGTGGCGCCCATGTGGAGCCGCCCCAGACGGGTCATCAGCCCCG GTGCGGTGCGGTGCAGCAGCAGCTCGCAGCCGCATCCGGCCAGCCCGGCGGCCGCGAAGACCCCTCCGGC CCCGGTGAGCGCCGCCACCGCCATGGTCACCAGACACCCGGTCAGCGCGGCGAAGGCCACGAGACCGCGG GGGCTTTCGATACCGAGGCGGTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 7301086-7303281	Sequence	M271_29760 - aromatic ring-opening dioxygenase LigA (Protein is coded in complementary strand; from 7303281 to 7301086)