DNA sequence >NC_022785, from 7284237 to 7284381 (145 bp); Streptomyces rapamycinicus NRRL 5491 GNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNN Translate to protein Restriction digest BLAST Design primers with primer3