Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 7227731
End position: 7228255
Length: 525

DNA sequence
>NC_022785, from 7227730 to 7228254 (525 bp); Streptomyces rapamycinicus NRRL 5491 ATGCCGGGCGAGCCGCCGCTGCGGCCGACCGCCGAGCCGGCCGCCATGGGCGACCCTTACGGCGACAACG CGATGGCGAACGCCCCTTACGGCGACCAGACGGCCGCCGGTGACCCGTACGCGGCGGTGCCGCACCAGCA GCAGTACGGGCAGCAGTGGGACGCGCAGACGTACGCCGACGCCGGATACGGCCAGTACCCGGCCGGTCAG TACCAGGGCGAGCAGTACGCGGGGGAGCAGTACCAGGGCGAGCAGTACCAGGGCGAGGGCTACGCGGGCC AGTACTCCACCGGCCAGTACCCGGCCGGTGACTACCAGGGCGGCTATCAGGACACCGGCTATCCGGCCGG CTACCAGGAAGGCCAGTACCCGGCGGACCCGTACGCGGCCGGCCAGTACGCCGACCCTTACGGCTACGAC CAGCAGCAGCCCTATGCCGACGGCGGCTACCCCCCGCAGGGCGACACGACGTACGGCGGCGCCGACCCCG AGGACCGCCGCGACGGGAGCGATCAGCAGCGATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 7227731-7228255	Sequence	M271_29500 - hypothetical protein
ORF... 7228252-7229841	Sequence	M271_29505 - hypothetical protein