Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 7219902
End position: 7221353
Length: 1452

DNA sequence
>NC_022785, from 7219901 to 7221352 (1452 bp); Streptomyces rapamycinicus NRRL 5491 CTACCGCACCACCACGAACCCCTCGACCCCCCGCTCCGGCCGCGCCACCGGCGGCACCGCCGCCTGGCCG ACCGCGACCGTGCCCATCGGGTCCCAGCCGTCCGGCAGGCCCAGGACCTCGCGCACCACGGGGCGGCAGA ACATCGTGGACGACACCCACGCCGAGCCCAGCCCCTCACCCGCCAGCGCGACCAGCAGGTTCTGCACCCC GGCTCCGGCCGCGACCACGAACATCTCGCGCTCCGCCGTGTTCCGCCGCGGATCGGGATACGTGTGGGCG CCGTCGGCTACCAGGCACGGCACCACCAGGTACGGCGCCTTCCGCAGCACATCCCCGCGCCGCACCCGCT TGGCGATGCTCTCCTCCGAGAAGCCGTCCCCGCGCAGATCGGCGATCCAGGCGTCCCGCATCGCGTCCAG CAGCCGCGTCCGCGACTCCTCCGACTCCAGCAGGACGAACCGCCACGGCGTCGTATGGTGCGGCGCCGGC GCGGTGACGGCCGCGGCGACCGCGCGCCGCACCGCCGCGCCGTCCACCGGCTCGTCGGTGAACTCCCGCA CCGTACGGCGCAGCGTCACCGCCTCCCGTACGGCCTCGGAGGTGCCCAGCCGGAACATGTCGTCCGCGGC CCCGCGCACCAACGCCCGCGCGCCCCCGTCCTCATCAACCCCCCGCCGCCCGGACCCCTCAGCCCCGTCC GGGCCCTCGGACTCCTCAGCCCCGTCCGGCCCCTCGAACGCGGCCACCCCGTCCAGCCCCTCGAACTCCA CCACCTGCGGAAGCCCCCGCAACACGGCCACCGGCAGCCCCGCGGCCTTGCCCTTGACCAGGTCACCCGC GGCGGCCAGTTCGTCAGCGGTGGCGACGATGGTCGCGCTCAGCGCGTTCCCGTACGCGTCGACTCCGCCG CGCAGATCGTCCAGGACCCGCACTCCGGCCGCGCCGATGGCGACATCGGTGACCCCGGTCCGCCAGGGCC GCCCGAACGTGTCGGTGACGATGACGCCGACCTCCACACCGAGCGCCTCCCGCAGCCCGGCCCGGATCGC CCGCGCCGAGGCGTCGGGGTCCTCGGGGAGCAACAGGATCGTCCCGGAAGGGGTGTTGGAGGCATCGACC CCGGCGGCGGCCATGACCAGACCGTGGCGGGTCTCGACGATCCGCGTACGGCCGCGCCGCGCCACGACCC GTACCGTCTCGGCGTCTATGGCCGCCTCGCGGTCGGCCGCCTCGACGACGCGCCCCTCCGCCTTGCTGAC GATCTTGGAGGTGACCAGCAGCACATCGCCGTGCGCGAGCCCCGGCAGACCGCCCGAGGTCGCGGCGTCG GCGATCAGCTTGGCGAGGTCGTCACCGGGCCGCACCTCGGGCATCCCGGGCAGCGCCCACACCTGGTACG ACTGGTACGACTGGGGCGACGCATGTGACGCATGTGACGCATGTTCCGTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 7219902-7221353	Sequence	M271_29475 - F420-0--gamma-glutamyl ligase (Protein is coded in complementary strand; from 7221353 to 7219902)
ORF... 7221350-7222312	Sequence	M271_29480 - 2-phospho-L-lactate transferase (Protein is coded in complementary strand; from 7222312 to 7221350)