DNA sequence >NC_022785, from 7218333 to 7218482 (150 bp); Streptomyces rapamycinicus NRRL 5491 CCACCNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3