DNA sequence >NC_022785, from 6923629 to 6923722 (94 bp); Streptomyces rapamycinicus NRRL 5491 NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3