DNA sequence >NC_022785, from 6843899 to 6844003 (105 bp); Streptomyces rapamycinicus NRRL 5491 GGTCGATCACAAGTGGGCCGACCGCAAGTGGGCCGATCACAAGTGGGCCGACCGCAAGTGGGCCGACCGC AAGTGGGTCGATCACAAGTGGGCCGATCACAAGTG Translate to protein Restriction digest BLAST Design primers with primer3