Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 6593081
End position: 6594619
Length: 1539

DNA sequence
>NC_022785, from 6593080 to 6594618 (1539 bp); Streptomyces rapamycinicus NRRL 5491 CTAATCCCCGCGGCCCTGCGACATGTTGATGCTCGCCCTGGCGACGCCACCGCTCTTCTGGCCGATGAGC TGGAAGGACACACCGCTCTCATCCGCCGGAGTGATCTTGTACGAGCCCACGGCCGCCGCCTTGCCCCGGC TGTCCACCTGGAAGGTCCCGATCTTGCGCTGGGCCTTCGAGCCCGAACCGGCGTAGGCGGTCACCGTCTC GCCCGGGGCGAACCCCGCGACATAGAAGCTGAGCGTGGTGCCCGCCCTGCCGCCGAAGGCGCTGGGCTCG GCGGACGGGCTGTACGGCAGCACGGTGAAGCCCGAGCGGACCACGGCCCGGCTCTGGTCGCCGATCGCCA TCAGCGTCTGCCGCCCCTTGAGGCCGAACGGCACGTTGAACGCCACGTCGCGGATCTGGCCCATGCCGTT CGCCTGGGCGGTGAAGGCCGGGAGGCCGCCGGTGGAGTTGATGTAGACCAGCACCCGCTCGCCGGGCGCG AACTTGTTGGCGGACAGCGTGATCCGCTGGCCGGCCTTGAGCGCGTACGGCTTGGACTTCATCGACGGGT ACATGGCCAGCATCTGGAAGGGCGCGGTGGCCGTGGTCTTGCTGCGCTTGCCCACCAGGACCAGGGTGCT GGAGCCGGTCGGGGCCACGCCCACCTTGATCGCCGCGTGGGCGACGCCGCCGCTGCCGTCGGTGTGCAGC GTGGTGACGGGGATGCCGGTGGTCCGGCCCCAGAAGACGTCGATCGGCTCACCCGGGCGGAAGCCGCGGG CGGAGACCGTCACCACGTCACCGGGGCGGCCCACCATCTTGTTGATCTTGGCGGTGCCGACGGCGCCACC GGTGATCACCTTGGCCTCCGCCGTCTTGCTGCTGCCGCGCTGCTGGGCGACGAGCGTCGCGGGCCGGTTG CCCGCGGTATCGGGCATGGTGAACCGGGCGTAGATCGACCCGAACCGGTCCGAGCGCACACTGCCGATGG CGCGGCCCTTGGTGTCCGACTTCCGGGCCTTGATCAGGATGTCCACGGTGGCCTTCTTGTCGAAGCCACC GCCCTCGACCGCGGCGCTGCCGCCGGGGGTGACCAGGCCCGGGTTGATCACGATGATCGGGCCGGACGCC TGGGGGACCCGCTGATTGCCCAGCTTGATGGTCTTGGGCTGCTTGGGTGGGCGTATCTCCTGCTCCTGCC GGGAGTGCTGCTCCTTCTTCGGGGCGCCGCCGCCTCCGCTGCCGGTGGTGTTCAGGGCGCCTCCGAAGCC GCCGCGCGCGACCACCTGGACGAGCGATCCGACGATCGCGAAGACCACGAGTGCGAGCAGGACGGTCTTC CAGGTGAGCTTTTTCTTCTTCTCACCGTCGGGAGTGTGCTCCTCGTCGAGGGTGTTTTCCTCGTCGGGGA TTTCCTCCTCGTCGAGGATGTCCTCCTCCTCGGGGATTTCCTCGTCGTCGAGGATGTCCTCCTCGTCGGG GATGTTCTCCTCGTTCTCGGGATAATCCCTGTCCTCCGGAACGTCCCAGTCGTCGTCGTACTGAGGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 6593081-6594619	Sequence	M271_26935 - aromatic ring-opening dioxygenase LigA (Protein is coded in complementary strand; from 6594619 to 6593081)