Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 6527516
End position: 6530380
Length: 2865

DNA sequence
>NC_022785, from 6527515 to 6530379 (2865 bp); Streptomyces rapamycinicus NRRL 5491 TTGTCCCCGACCACCAGCGAGACCGCGCAGGGCGGCCGCCGACTCGTCATCGTCGAGTCGCCTGCCAAGG CGAAGACGATCAAGGGCTACCTCGGCCCGGGCTACGTGGTCGAGGCCAGCGTCGGGCACATCCGGGACCT GCCGAACGGTGCGGCCGAGGTCCCCGACAAGTACACCGGCGAGGTCCGGCGCCTCGGCGTGGACGTCGAG CACGACTTCCAGCCCGTCTATGTGGTCAACGCTGACAAGAAGAGCCAGGTCAAGAAGCTCAAGGAGCTGC TCGCCGACTCCGACGAGCTCTTCCTGGCCACCGATGAGGACCGCGAGGGCGAGGCCATCGCCTGGCACCT CCAGGAGGTCCTCAAGCCCAAGGTCCCGGTGCGCCGGATGGTCTTCCACGAGATCACCAAGGACGCCATC CGGGCCGCCGTGGCCAACCCGCGCGAGCTGAACCAGCGCCTGGTCGACGCGCAGGAGACCCGCCGCATCC TCGACCGCCTCTACGGCTACGAGGTCTCGCCGGTCCTGTGGAAGAAGGTCATGCGCGGCCTGTCGGCGGG CCGGGTCCAGTCGGTCGCCACCCGTCTCGTCGTCGAGCGCGAGCGCGATCGCATCGCCTTCCGCTCCGCC GAGTACTGGGACCTCACCGGCACCTTTGCCACCGGCCGGGCCGGTGACGTCAGCGACCCCGGCACCTTCG GTGCCCGCCTTACGGCCGTCAACGGCCTCCGGGTCGCCCAGGGGCGCGATTTCACCTCGCTCGGGCAGCT CAAGGAGGGCCTCAACGTCCTCCACCTGGACGAGGCGGGTGCCCGCTCCCTGGCCGCGGCCCTCGTCGAC ACCGCCTTCGCGGTGCGCTCGGTCGAGTCCAAGCCGTACCGCCGCTCGCCGTACGCGCCGTTCCGCACCA CGACCCTGCAGCAGGAGGCCAGTCGCAAGCTGGGCTTCGGTGCCAAGGCCACCATGCAGGTCGCGCAGAA GCTGTACGAAAACGGCTACATCACCTATATGCGTACGGACTCCACCGTCCTGTCGGACACGGCGGTGGCG GCGGCCCGGGCGCAGGTCACCCAGCTGTACGGGGCCGACTACCTGCCCGACAAGCCGCGCACCTACGCGG GCAAGGTCAAGAACGCCCAGGAGGCGCACGAGGCCATCCGCCCCTCGGGAGACCGTTTCCGCACCCCGGC GGAGACCCGCCTCACCGGCGACCAGTTCCGGCTCTACGAGCTGATCTGGAAGCGGACCGTCGCCTCCCAG ATGAAGGACGCGATCGGCAACTCGGTCACCGTCAAGATCGGCGGGCAGGCGAGCGACGGCCGGGACGCCG AGTTCTCCGCCTCCGGTAAGACGATCACCTTCCACGGCTTCATGAAGGCCTATGTGGAGGGCGCCGACGA CCCGAACGCCGAGCTCGACGACCGCGAGCGCCGGCTGCCGCAGGTCACCGAGGGCGACCGGCTCACCGCC GAGGAGATCACCGCCGACGGCCACGCGACCAAGCCCCCGGCCCGCTACACCGAGGCGTCGCTGGTCAAGG AGCTCGAAGAGCGCGAGATCGGCCGCCCGTCGACCTACGCCACGATCCTTGGCACCATCCTCGACCGCGG CTACGTCTTCAAGAAGGGCACCGCGCTCGTGCCCTCCTTCCTGAGCTTCGCCGTGGTCAACCTGCTGGAG AAGCACTTCGGCCGGCTGGTCGACTACGACTTCACCGCCAAGATGGAGGACGACCTCGACCGCATCGCGC GCGGCGAGGCCGAGGCCGTGCCGTGGCTGAAGCGCTTCTACTTCGGCGAGGGCGCCCAGGTCGGCGCCGC CCAGGCCGGTGCCGCCGAGGCCGGGAACGGCGACGGCGACCACCTCGGCGGCCTCAAGGAGCTCGTCACC GACCTGGGCGCGATCGACGCCCGGGAGATCTCGTCCTTCCCCGTGGGCGACGGCATCGTGCTGCGCGTCG GCCGCTACGGGCCGTACGTCGAGCGCCCCACGGAGGTCGAGGGCGGCATCGGCCAGCGCGCCGACGTCCC CGACGACCTGCCGCCGGACGAGCTGACCGTGGAGCTCGCGGAGGAGCTGCTCGCCAAGCCGAGCGGCGAG TTCGAGCTGGGTGCCGACCCCGAGACCGGGCGCATGATCGTCGCCAAGGACGGCCGCTACGGCCCGTACG TCACCGAGGTGCTCCCCGAGGACACCCCCAAGAGCGGTAAGAACGCCATCAAGCCGCGCACGGCCTCCCT GCTGAAGTCGATGTCCCTGGACACGGTGACCCTGGAGGACGCGCTCAAGCTGATGTCGCTGCCGCGTGTG GTCGGCACCGACCCCGAGGGCGTGGAGATCACCGCGCAGAACGGCCGCTACGGCCCGTACCTGAAGAAGG GCACGGACTCGCGGTCGCTCGAGACCGAGGACCAGATCTTCAACATCACGCTCGAGCAGGCGCTGGCGAT CTACGCCCAGCCCAAGCAGCGTGGCCGGGCCGCGGCCAAGCCGCCGCTGAAGGAGCTGGGCACCGACCCG GTCAGCGAGCGGCCCGTGGTGGTCAAGGACGGCCGCTTCGGTCCGTACGTCACGGACGGCGAGACCAACG CGACGCTGCGCCGCGACGACGACGTGGAGACCATCACATCCGAGCGCGGCTTCGAGCTGCTCGCCGAGAA GCGCGCCAAGGGCCCGGCCAAGAAGACCGCCAAGAAGACGGCGGCGAAGAAGACCGCGGCCAAGAAGACG ACGGCGAAGAAGACGGCCGCCAAGAAGACCGCGGCGACGAAGACGACCGCGAAGAAGACGGCCGCCAAGA AGACGACCGCCAAGACCGCCGCGAAGAAGACGGCGGCCGAGAGGTCGTCGGCCAACGCCGAGTAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 6527516-6530380	Sequence	M271_26585 - DNA topoisomerase I