DNA sequence >NC_022785, from 6515412 to 6515586 (175 bp); Streptomyces rapamycinicus NRRL 5491 TGGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3