Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 6455229
End position: 6456488
Length: 1260

DNA sequence
>NC_022785, from 6455228 to 6456487 (1260 bp); Streptomyces rapamycinicus NRRL 5491 ATGACCAGCACCACCCCACCCGCGCCGTCCATCGACGGCGCCGCCCTGCTCAACGAGGTCGAGCAGCACC ACCGGCGCTTCAACGTCTTCCCCAGCGAGCACGCGTACGTGGCCGTCACGCTGTGGGACGCCCACGCGCA TCTCATCAGCGCCTTCGACTCCACCCCCAGGATCGCCTTCCTGTCCCCCGAACCCGGCTCGGGGAAGTCC CGCGCGCTGGAGATCGTGGAGACCCTGGTTCCCAACCGGCTCGTGGCCGCGCACGCCACCGCCAACGCCA TGTACCGAGCCGTCGGCGACACCGACAACCTGCCCACGGTCCTGTTCGATGAGGTAGATACCATCTTCGG CCCCAAGGCCGGGGACAACGAGTCCCTGAGGGGCTTCATCAACGCAGGACACCGCCGGGGCGTCGGCGTC CTGCGCTGCGTCGGCGACGGCTCCAACCAGGAAGCGACTGAGTTCCAGACCTTCTGTGCCGTGGCCATGG CCGGACTCGGCTCGCTCCCGGACACGATCCTGACCCGCTCGGTCATCATCCGCATGCGCAAGCGCGCACC CAACGAGGACATCCAGTCCTACCGGCAGCGTGTCCACGAGAAGCAGGGCCACGCCCTGCGGGACCGGCTC GCCAAGTGGGCCGACCAGGTGCGCGACCAGGTGGAGAACGCTTGGCCGGAGCTGCCCGAGGGCATCAGCG ACCGGCCCGCGGACGTCTGGGAGCCCCTGCTCGCCGTCGCCGACGCGGCCGGCGGCAACTGGCCCGAGCG GGCCCGCGCCGCATGCCTTGAGCTGGTCGCCGCGGCCAAGGAAGGCGACAAGGCGTCCGTAGGCGTGCAA CTGCTGACCGACCTGCGGGACAAGGTGTTCTGCGGCGCGGACCGCATGCCCACCGCCGCGATCCTGGAAT GCCTCCACGCGATCGAGGACGCCCCGTGGGGCGACATGGACGGCAACGGCAAGGAACTCACCCCCCGGGT GCTGTCCAAGATGCTGGGCGAGTACGTCACCGCAGCCAACAAGCCCATCCGGCCCCGCTCCATCCGCACG GCCGGCGGGGTGCCCAAGGGGTACTACGCCGAAGACCTCGCCGACGCTTGGTTGCGCTACTGCCCCCCGG CCCCGGAGAAGTCCGCTACATCCGCTACATCCGCTACACCGCAGGTCAGCAGGGGTGAAAACGTAGCGGA TCCGCTTCCCGACATCCGCTACATCACCGACCGGCCCGCTACACCCGCTCTCGGCGTCCGTCGCGCATAG

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 6454858-6455232	Sequence	M271_26250 - hypothetical protein
ORF.. 6455229-6456488	Sequence	M271_26255 - hypothetical protein