DNA sequence >NC_022785, from 6259536 to 6259710 (175 bp); Streptomyces rapamycinicus NRRL 5491 GACCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3