Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 6221314
End position: 6222294
Length: 981

DNA sequence
>NC_022785, from 6221313 to 6222293 (981 bp); Streptomyces rapamycinicus NRRL 5491 TCAGCGCCGCGGGTAGGTCCGGGTCACCAGGAGCTCCCCGGGAGTCGTCGGGGACGCCGAGACGCAGGAC GCGTCGATCACGAAGGCATCGACGCGCGAGCTGTCGGAGGGGTGCGGGAGGACGACGATATAGGCGCTCT GGCCGTCGTAGATGTCCTGCTGTGCCGCGAGAGCCGGTTCCTTGCGGCCGGTGCCGCTCTGTACGCAGGA GGGCACCTCAGGGTCGTCATCGGCTCGCAGCGGCACATCGGGTGAGTTGCCCTGCCCGGTGACACCGTTC GACGCGGTGGACTTCCGGTCGGCGAGCAGTTCGTGCACGCGGTCCTTGAGCGTGCCCGAGGAGAGGGTGG AGTTCCCGGTGGAGCTCTGGCTGGCGTGCGCCTGGGTGTCGCTGTCGCTGGGATCGCCGGAGGAGCCGCC CTGGAGGAAGAAGGACCCGATGCCGATCACCGCCGCGGCGCATGCGCCGCCGAGGACGGCCTTGCGCCAG CGCCGGGCGCGGCCGGGGCGGGTGCCGGGTCCGGTGGTTCCTCGTCCTGGCCCAGTGGCCCCACGCGGAC GCCCGGCGGGCCGGTCCGCCGCGATGGACGGCTTGGCCGTGGGCTCCACGGTGTCGGAGCCGGGGTCGCT GGAGGCGTCATCGGCCGTTTCACGTGAAACAGGCGGGTGCGCGGCGTCCGGATCCGGAGTTTCACGTGAA ACATCGGCCACGCGCTCGGGCGCGGTCGCGTCGAGGAGCGCTTCGGCGGCCAGTGCCGCGTCGATGCGGC CCGCGACATCGGCGGGCATCCGTACCGGCCCCGGGAGGGTTCCGAGCAGGGAGCGGATCTCGTCCAGGGA CTCCCATACGTCCGCACACAGCACGCAGCTCGAGAGGTGAGTGCGCACATCGACGGCGCGGGACGGAGAG AGCAGACCCTCGGTGAGTGCGGAGATCTCCGCGACTTCAGGGTGCTCTTCCGTGTCCGTCGAGGATGTCA C

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 6221314-6222294	Sequence	M271_25195 - membrane protein (Protein is coded in complementary strand; from 6222294 to 6221314)
ORF... 6222291-6222980	Sequence	M271_25200 - RNA polymerase sigma factor SigM (Protein is coded in complementary strand; from 6222980 to 6222291)