Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 6156456
End position: 6157607
Length: 1152

DNA sequence
>NC_022785, from 6156455 to 6157606 (1152 bp); Streptomyces rapamycinicus NRRL 5491 CTACAGCCGCCGCAACAACACCATGGGCTGCTCCACGCAGTCGGCCACGAACCGCAGGAATCCGCCTGCG GTGCCCCCGTCGCACACCCGGTGGTCGAAGGTGAACGACAGCTGAACCACCTGGCGCACCGCCAGCCCAC CCTGGTGCACCCAGGGCTTCGGGGCGATCCGCCCGACCCCGAGCATCGCCGCCTCGGGGTGGTTGATGAT CGGCGTCGAGCCGTCGACCCCGAACACCCCGTAGTTGTTCAGGGTGAACGTGCCGCCGGTGAGCTGCGCG GGGGCGAGGGTCCCGGTCCGCGCAGCCTCGGTCAGCCGGGCGATCTCCTCGGAGAGCCCCTCTATGGTCC GCGTGTGGGCATCGCGCACCACCGGGACGACCAGGCCCCGGTCCGTCTGCGCCGCGAAGCCGAGGTGGAC GTCGGGGAGCCGGACGATCTCCCGGCCCGCCGTGTCCACCGTGGCGTTGAGCTCGGGGAAGCGCGCGAGG GCCGCCGTGCAGATACGGGCCAGGAGGGCGAGCACGGAGACCTTGGCCTCGCCCGTGGCGTTCATCGCCG CGCGGGCGGCGAGCAGTTCGGTCGCGTCGGCGTCCACCCAGCAGGTCGCGTCGGGGATCTCGCGGCGGCT GCGCGCGAGCTTGTCGGCCACGGCGCCCCGCACCCCTCGCAACGGAACCCGCTCCCCCTCCGGGACGAGG CCCGCGGAAACNNNNNNNNNNNNNNNNNNNNNNNNNGGCAGCGGTCCACCGGGGGCCGTGGGCATCTCGG GCGGCGGCCCGGGGGAGGGCCGCACGCGGCGGCGCCGCGCGGCGGGACCGGCCGTGCCATAGCCGACCAG CACATTGCCCGACGCGCCGTCGATCACGCTCTCAGTGACCGGCGGACCGGATCCGTCGTGCGAGCCCTCG GGCACGGCGACCGTGATCAGCGGCGCCCCGACCGGCACCTCCGCGCCCTCCTCGCCGTAACGGGCCGTCA CCACACCGCCGTAGGGGCACGGCACGTCCACCATCGCCTTGGCCGTCTCGACCTCGACCACCGGCTGGTC GACCGCGACCACATCGCCGACCTGGACCAGCCAGCGCACGATCTCGGCGCCGGTCAGCCCCTCGCCCAGA TCGGGCAGGGTGAATTCCCGTACGACAGCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 6156456-6157607	Sequence	M271_24880 - branched-chain alpha-keto acid dehydrogenase subunit E2 (Protein is coded in complementary strand; from 6157607 to 6156456)
ORF... 6157607-6158656	Sequence	M271_24885 - 2-oxoisovalerate dehydrogenase subunit beta (Protein is coded in complementary strand; from 6158656 to 6157607)