Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5550199
End position: 5550831
Length: 633

DNA sequence
>NC_022785, from 5550198 to 5550830 (633 bp); Streptomyces rapamycinicus NRRL 5491 TCATGCCTGCACCCCCTGCTGCCGCTCCGGCGACTCCTGCGACTCCTGCGACTCCTCGTTCCCCGCGGCC TGGTCGGACAGGGTCTCGCCCTTGCTGACCGCGACCTGCTGGGTGGTGCCGGGCGCGGCCTCGGTCACCA GGCCCCGGTAGTAGTCCTGCTCGTCGGTGCCGGGGTAGATGGCGTGCGGGGAGTCGACCATCCCGTCCTC GAGGCCCGCGAGCAGCTCCTCCTTGGTGTAGATGAGCGATTCGCGGGTGCGGTCGGCGAGCTCGTACTCA TTGGTCATGGTCAGCGCCCGGGTGGGGCACGCCTCGACGCACAGGCCGCACAGGATGCAGCGCAGATAGT TGATCTGGTAGACGCGGCCGTAGCGCTCACCGGGCGAGTAGCGCTCCTCGTCGGTGTTGTCCGCGCCCTC CACATAGATCGCGTCGGCGGGGCAGGCCCAGGCGCACAGCTCGCAGCCGATGCACTTCTCCAGCCCGTCC GGGTGCCGGTTGAGCTGGTGGCGTCCGTGGAAGCGGGGCGCGGTGGGCTTCTTCACCTCTGGGTACTGTT CGGTGAGCCGCTTCTTGAACATGGCCTTGAAGGTCACGCCGAAGCCGGCCACCGGATTCTGGAAGTCAGG CAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5549279-5550202	Sequence	M271_22080 - NADH:ubiquinone oxidoreductase subunit J (Protein is coded in complementary strand; from 5550202 to 5549279)
ORF.. 5550199-5550831	Sequence	M271_22085 - (4Fe-4S)-binding protein (Protein is coded in complementary strand; from 5550831 to 5550199)
ORF... 5550824-5552182	Sequence	M271_22090 - NADH:ubiquinone oxidoreductase subunit H (Protein is coded in complementary strand; from 5552182 to 5550824)