Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5454892
End position: 5458116
Length: 3225

DNA sequence
>NC_022785, from 5454891 to 5458115 (3225 bp); Streptomyces rapamycinicus NRRL 5491 TCATTCACCGCCGGACCGCAGGAATTCCGCCAATTGGGCCGGGCTGGGCCTCGTGAAGATTTCGGCGAGC TGGAGACGTACGCCGAGCCGTTCCTCGACGGACTGGGCGAGCTTCGCCGCCAGCAGGGAATGGCCGCCAC TGGTGAAGAAATTGGAATGGGCGTGCAGATCGGTACGGCCGAGCACGTCCTGCCACAGGTCGATGAGTTT CCCCACGAGTTTGTCCTCCGGTTCGACCGCGGACGGCTCGGCCCGTTCCTGGTCGCGGTCCCGTATCCGG CGGGCCAGCGCCAAATAGTCGACCTTGTGGTTCCCGGTGGTGGGCAGGGCGCCGACGAGGAGGAATTCCC CGGGGACCGCGGGGGCCGGGAGGCTGCTGCGGGCGTGCTCCCAGACCTGGTTGACCAGGCCGGGGACGTC CTCGGCGAGGAGGAACGCCACCAGCCGGGCGTCGCCCGCCTCACCCGTGACGACCAGGGCGGCCGCCGCC CGGACGGCGGGGTGGCCGAGGAGCACGCTCTCCACCTCGCCCAGCTCGATCCGGTTGCCCCGCAGCTTCA CCTGCCGGTCGACGCGGCCGAGCACCTCCAGCGTTCCGTCTCCCCGCCACCGGGCGAGATCACCGGTGCG GTAGTAGCGCCCGTGGCGGGGGTGCTCCCGGAACCGGTCGGCGGTCAGCTCCGGGCGGCGGTGGTAGCCG ATGGCCACTCCCCCGCCGGCGACGCACAACTCGCCCCGCACGCCCACCGGCAGTTCCGCACCGTCGGGGG TGGCGACGAACACCCGGGTGTCACGGATCGGCCGGCCGACGTGCACCGGGCCCCCGGGGCCCGGCTCGAC CAGGCCGGATGTCGACCAGATGGTGGTCTCCGTCGGTCCGTAGACATTGCGCAGCTCACAGCCGGTCGCG GCCAGCCGGGCGGCCAGCGCCGACGGGAGCGGTTCGCCGCCGCACAGGACGCGCCGCCCGTCCAGTTGGT CCGCGCAGTCGTCGGTCACCAGCCGCCAGGTGGTCGGGGTGGCCTGGACGATGTCGACCCGGTGGTCCCG CAGCAGATCGGCCAGGACGCGGCCGTCGGACCGTGCGGCGTCCGGCGCGGCCACCAGGCGCCCGCCGGTG ACCAGCGGCAGGAACAGTTCCAGGGCGGAGATGTCGAAGCTGAAGGTGGTCAGCCACAGCGTGGTCTCAC CCGGCCGGGCCCGGAGTTCATCGGCGAAGTGCCCCACCACGTTGGTGAGGTTGCCGTGGGAAACCAGGGT TCCCTTCGGCCGTCCGGTCGAGCCTGAGGTGTAGATCAGGTAGGCGGCGCGGGCCGGATCGGGATCGGCG CCCAGGTCGGAGCCGGTACGAGGGTCGGGGTCAGTGCGAGGGTCGGGGTCAGTGCGAGGGTCGGAGCCGG TGCGGGGGTCGGAGCCGGTGCCGAGCGCGGAGTCGGCGCCGGAAGCAGGGTCACTGCCGGTGGCGGGCCG GGTGTTCAGGTCGGCCAGGCGCAGCACCGGAACCCCTTCGGGTGCGGGCACGGACCCGTCCCCGGCCAGA ACCGCCTTCGCCCCGGAGTCCGCGAGCTGGTACGCGAGGCGGTGCGCGGGGTGGTCGGGGTCCAGGGGGA GGTAGACCGCGCCGGTGAGCCATACGCCGAGCACGGCCGCGGCCAGCGCGGCCGAGCGTGGTGCGATCAG CGCGACCACATCGCCGTCGCCGATGCCGTGGTCGCGCAGCGCACTCCTGACGGCGAGGGCCGAGTCCCAC AGCTCCCGGTAGGTCCGGGGACCGTCCGCGTCCTCGACGGCCACCGCGTCCGGTGTCCTCGCGACGGTCC GCCGCACTCCGGCGAGCACGGAGGGCGCGGGCCGGTCCGGCGGGGGCTGACCGGCGGCGTCGATCACGGC GTGGTCGCGTTCGCTCCAGGCCACGGCCCCGCCGAGGGGCTGCCCGGGGTCCTCGGCCAGGGACGACAGC AGCACCTCGTAGCGCCCCAGCAGCGCGGCGACCTCGTCACGCGTGAACACATCGTCGCTGTAGACGATGC GCAAGCGCAGTTCCTCGTCGGTCGACATGACGAAGAACTCCAGGTCAAACTTGCTGTACGGGATCTCGAC GACCATGGGGGCGGCGGCCAGGCCGCCGATCTCGAAGTCGGGCAGGCCCAGACCCGGCACGTAGTTGAAG ACGTGCCGGAAGAGGCTGTTGCGCCACGCGCCGCCCTCTCGGGCTACTTCGTTGTAGAGGCTGTCCACCG GCACGTCGGCGTGGGCGATGCCGTCGAGGAAGACCCGCCCGGCCTTCAGGGCCAGTTCCCGGAAGCTGTC GTCGGGGTCCACCGTGAGCCGCAGGGGCACCACGTTGACGTGGTAGCCGATCGCGCCGGGCGCGGAGCGG CCCCGTACATTCACCATCGTGCCCACGGCCAGGTCCGGCCCGGCCCCGTGCTGGGCGAGCAACAGACCGT AGGCGGCGAGCAGGACGACGGCCTCGGAGGTGTTCAGTTCGGTGCGCAGCCGGTCCACCGCCGCCCGGAC CCGGCGGGGCACGGTGACGGTCAGCTGGCCACCGGCCAGGGTCGAACCGGCGGCCTCCTGCCTGCCGTAG GCCAGCTCGAGCCTGGAGGGATCGAAGCCCTGGAGCTGCCGGTGCCAGTAGCTGAGGCTCTCGGCGGTCG GCTCCGCCTCCCGCAACGCCGGCACCGGCTCCAGCAGAGCGGGGTCGGGCGGCCGGTTCGCGGCGAGCGC GGTGTACACCTCGACCAGCTCGCCGAGGAGGATGCCCGCGGAGATGGTGTCGAAGACCAGGTGGTGCAGG ACCAGGCAGACGATGTCCTCACCGTCGCCCGGCAGGTGCCCGACGCGCGTCAGCGGCCGGCCGTCGAAGG TGAACGGCATGGCCACGAAGGAGGTCAGCGCCTGCTCGGGATCGCCCGAGGCCGGTACGGTGAGCACCTC CGCACCGGCCTCATCGACGTCGAGGACGACCTTGGTCAGCCCCGTGTCGCCGGCGTGGAACACGGTGCGC AGCGTCTCGTGGCGTCGCACGAGCAGCGTCACGGACGCCCGCAGGATGGACCGGTCCAGGCCGCCGCGCA CCCTGAAGGTGAAGGACAGGTTGTTCACGGCGGCGCCGGGGACCAGCCGTTCCAGCAGCCACAGCGCCTC CTCCTTCCGGGTGACCGGTTCCGGCCGCGGCGCCCTTCCGGTGCCGCTGGGGCCCGGGTCCGTCCGTGTC CGCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5453597-5454895	Sequence	M271_21650 - hypothetical protein (Protein is coded in complementary strand; from 5454895 to 5453597)
ORF.. 5454892-5458116	Sequence	M271_21655 - hypothetical protein (Protein is coded in complementary strand; from 5458116 to 5454892)