Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5435715
End position: 5437112
Length: 1398

DNA sequence
>NC_022785, from 5435714 to 5437111 (1398 bp); Streptomyces rapamycinicus NRRL 5491 TCAGGCTTTGCCGAACTCCTGGTGGATGAGGTCGAACAGGTCGTCCGCGGTGGCCCCGTCCAGTTCCTCG TCCCGCGCGTGGTCGTCGTCGGTGTCCCGGGCCGGTCCGGGCCGTGCCGCCGAGGGGCCGGTGCGGTCCG GGAGGAGCGCGGACAGGCGTTCCCGGACGGTGGTGTGGGCGGGGTCGTCGGCGGGGAGCACCGCCAGCGC GGACTCCAGCTCCTGGAGCGTGCGCAACGCCCGCCCACCGGAGCCGGAGCCGGAGCCGGAGTCGGGCCCG GAGCCGGAGTCGAGGATCTCCGTCCGCAGATGGCGGGCGATGCTCTCCGGGGTGGGGTGGTCGAAGGCGA GCGCGGCGGGCAGTCGCAGACCGGTGGCCGCGACCAGCCGGTTGCGGAAGTCGACGGCGGTCAGCGAGTC GAAGCCCAGGTCGCGGAAGGCCCGGTCCGGATCGACGGTACGGGCCGAGGGGTGGCCCAGAGCCGCCGCG GCGTGCTCGCGCACCAGGTCCAGCACCAGTTGGTCCCGCTCCGGCTCGGGCGCGCGGGCCAGTCGTTCGG CCAGCGTGGGCCCGGACGGGTCCGGGGCGACGGCCACCGTTTCCTCCGCCGTCCGCCGCACCTCCGCCAG GTCGCCGATCAGCGGGCTCGGGCGCAGCGCGGTGAAGCCCGGGGCGAACCGCTCCCAGTCCACATCGGCC ACGACCACACAGGCCGGGCCGCCCGGCGCGTCCCACAGGGCGTCCACCGCGAGCCGGGGGCTGAGGGTCC GCAGACCGCGGCGGCGCAGGTGGTCCTCCGCCGCGGCGGCCATTCCGCCCTCGGCCCAGGGCCCCCAGGC CACCGAGGTGGCGCACCGGCCACGGTCCCGGCGCTGCTCGGCCAGCGCGTCGAGGAAGGCGTTGGCCGCC GCGTAAGCGCCCTGTCCACCGCTGCCCCAGACGGCGGCGATGGACGAGAACAGGACGAAGGCGTCGAGGG GTTCGTCGGCGAACAGCTCGTCCAGGTGCACCGCACCGGTGGCCTTGGCGGACACCACCGCGTCGTAGTC GCCGAGGTCGGTGCGTACGTCCGGCATCTCGGGCGCTCCGGCGGCGTGGACGACGGCGGTGACCGGCGGG CCGGCGTCGCGGAGCCCGTCGACGAGGGCGGCCAGGGCGTTCCGGTCGGTGACGTCGCAGGCGGCCATGG TGACCGCCGCGCCCAGGCCGGTCAGTTCGCCCTCCAGGGCGGCGGCACCCGGCGCCGACCGGCCTCGGCG GCTGACGAGCACCAGGTGCTCGGCGCCCTCACGGGCCAGGGCGCGGGCGACGTGTGCGCCCAGGGTGCCG GTGCCGCCAGTGATCAGTACGGTGCCTCGTGGCCGCCACCGAGGTGTCCGGGGGGGCCTGTCCGTCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5435715-5437112	Sequence	M271_21635 - hypothetical protein (Protein is coded in complementary strand; from 5437112 to 5435715)