Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5401312
End position: 5404821
Length: 3510

DNA sequence
>NC_022785, from 5401311 to 5404820 (3510 bp); Streptomyces rapamycinicus NRRL 5491 CTAGTGGCCCAGTTCGGTGTCGATGAATTCGAAGATCTCGTCGTCGGTCGCCGAGTCGATCCGCTCCGCG ACCGTGGTGTCACTGATGGCTCCGCCCGCCCCGTCCACCATGGACAGCAGCTCCTGCAGCCGCACGGTGA GCCTCGCGCGGGCCACGTCGTTCCGGGACAGCGCCGCGAGGTCGTCCTCGATCCGGTCCAGCTCCGCCAG CAACGGCAGGACGGACGCGGTGCCGTCCGGCACGATCCGCTCGGCGAGATGGCTCACCAGCGCGGACGTG GTGGGCTGATCGAAGATCAGCGTGGCGGGCAGCCGGAGGCCGGTGGCGGCGGTCAACCGGTTGCGGAACT CGACCGCGGCCAGCGAATCGAAGCCCAGCTCGCCGAAGGTGCGGTGCGGATCGACGGCGGCCGACGAGGC GTGCCCGAGGACGGCGGCCACCTGCTCACGGACGAAGTCGGAAAGTTGGAGCTCCCGCTCGGCCTCGGAG GCCCCGGCCAGCCGTTGCGCGAGCGAGGGCACCGAGCCGTTCGCCGCCGTACCCGCCGCCGGGACCGGTA CGAGCCCGTGGAACAGCGGCGGGAGCAGTTCGGCGGGCCGCGCCCGCAGTGCGGCGAGGTCCAGTCGGAT CGGCACCGCCAGCGGCCGGTCGGTGGACAGCGCGGCGTCGAGCAGCCGCAGCCCCTCCGCATCGGCCAGC GGGACCACTCCGGTCCGGCTCATCCGCCGCCGGTCCGTCGCGTCCAGCGTGCCGGTGATGCCGCTCTCCG ACGCCCACAGCCCCCAGGCCAGCGAGGTCCCGGCCAGCCCCGCCGCCCGGCGCCGCTGGGCCAGGGCGTC CAGGAAGGCGTTGGCGGCGGCGTAGTTGGCCTGTCCGGCCGCGCCCATCGTGGCGACGGCGGAGGAGAAG AGGACGAACCGGGAGAGGGGCAGGCCCTTGGTGAGGGCGTGCAGATTCAGCGCGGCGTCCACCTTCGGGC GCAGCACTCGGTCGAGCTGTTCGGGTTCCAGGGTCTGGAGGACCCCGTCGTCGACCACGCCGGCCGCGTG CACCACACCGGTCAGCGGGGCCTCTTCGGGGATCCGTGCCAGCAGCCCCGCGAGCGCTGCCCGGTCCGCC ACATCGCATGCCTCGACGGTCACCTGGGCGCCCGACGAAGTGAGTTCCGCCGCCAGTTCGGCGGCTCCCT CGGCCGCCGGGCCCCGGCGGCTGACCAGCAGCAGACGGCGTACGCCGTGTTCGGTGACGAGGTGGCGGGC GGTCAGCGCGCCCAGGGTGCCGGTGGCGCCGGTGATCAGTACGGTCCCTCGCGTGTCCCACGGGGACGGC TGCCGCGGCCGCAGCCGGGCCAGCCGGGGCACGAACGCGACCCCGCCACGCATCGCCGCCTGCGGTTCCT CCGCCGCGACCAGCGACATGAGGAGCTGGGGGAGGGCGCGGCGCGAGGTGTGCGCGTCATCGAGGTCCAG CAGGGTGAAACGGTCGGGGTGCTCCGTCTGCGCCGTCCGTACCAGGCCCCACAGGGCCGACGCGGCCAGA TCCGCCACACCGTCCCCGGGCCGGGCGGCCACGGCGCCGCGGGTGACCAGAACCAGGCGGGTGCCCGTGA AACGCTCCTCCGCCAGCCAGTCCCGCAGCAGCCGCAGCACACCATGTGCCGTGTCCCGCGCGGCGTCCGC GGCAACTGTCTCGGATGCGGTCGTGGATGCGGTCGCGGAGGCGGGGCCGGAGGCGGGGCCGGACGGAACG GAGTGCGAGACACAGGACGCCACCACCACGTCGGGCGCCGCGGCCTGGGTCGATGCCTCGGCCAACGCGC TCAGGTCCGGGAAGGAGCGTACGGACACGGAGGCGTCCGTCAGCGTCTCGGCCAGCCCGAGGCCGTCCGC CCCGAGCACACCCCACCATCCGGCCGCGGGCGGTGCGGGCTCCGGCCGCGGCTCGACCCAGTCCACACCG AACAGTGCGTCACGGTGGTGCGACACTCCGCCGACGGCGGCCAACTGCTCCGGCTTCACCGGCCGCACGG CCAGGGAATCCACCGTGATCACCGGGGCGCCGGTGCTGTCGGCGATGGACAGCGCCACCGCTTCGTCGCC TACGGGGGACACCTGGACGCGGAGCGCGGAAGCGCCCCTGGCGTGCAGGGTGACGCCGTTCCACTGGAAC GGGAGCCGGATCCCGGCCTCGTCCGGCGTACGGTCGGCGGCGTCGAGGGCAGCGGCATGCAGGGCGGCGT CGAGCAGCGCGGGGTGCAGGGCGAACGACGCGCTGTCGGTGCCGGGGGGCAGTTCGACCTCGGCGTAGAT CTCCTCCCCCAGCCGCCACGCCGCCCGCAACCCCCGGAACACCGGCCCATAGCCATACCCCAGACCGGCC AACCGCTCATACAACCCATCCACCACCACCGACTCCGCACCCTCCGGCGGCCACACCCCCACACCCACCG ACCCAGCCACCCCAACAGCCCTCAACACACCCGACGCATGACGCACCCACGGCACCCCCGACGACACACC CTCCTCACACGAGAACACCTCCACCGACCGCGCCCCCACCTCATCAGCACCACCCACCACCACCTGCAAA CGCACCCCACCCCCATCAGGCACCACCAACGGCGCCTCCAACGTCAACTCCTCCACCCGACCACACCCCA CCTCGTCACCAGCACGCAACGCCAACTCCACACACGCCGCCCCCGGCAACAGAACCCGGTCCATGACCTG GTGGTCGGCCAGCCAGGGAAGGGCCGATACGGAGAGCCGCCCGGTGAAGAGGTACTCATCACCGCTGGAG AGCGCGACCGCCGCCCCGAGCAGCGGATGTCCGGCCGAGTCGAGCCCGGCGGAGGACACGTCCATGGGAG CCGCGGCCTCCTCCAGCCAGTAGCGCTTCCGCTGGAAGGCGTAGGTGGGCAGGGGGATGACGCGCGATCC GGCGAAGACCGCGTGCCAGTCGACGGCGGCACCGCGCACATGGGCGGTGGCCAGTGCCCGCCACAGCGTC TCGGGTTCGGGCCGGTCGCGGCGCAGGACGGGAACGCTCACCCCGGCCCCGGACACGCCGTCCTGGACCA TCCCCGACAACGTCCCGTCCGGACCCAACTCCACGAACGTGCCCACACCCTCAGCTTCCAGGGCACGGAG ACCGTCGTGGAAACGCACCGCCTCACGTACGTGCCGGACCCAGTACTCCGCACCCTCCGGAACACCCACC CGGCCCGACACATTCAGCACCAACGGAATCCTCGGTGCCTCGTGCTCCACCGACTGCGCCACCTGCCGGA AATCCTCCAGCATCCCTTCCATCAGGGAGGAGTGGAACGCATGCGAGACCCGCAGCCGACGCACCCGCCG ACCCTGAGCCTCCCACTGCGCCGCGACCGCCTCCACCGCATCCCCCACACCCGACACCACCGTGGACACC GGACCATTGACCGCCGCGACATCCACACCCTCCGGCAGGCCTTCCAGCACCTCCGCCTCGGACGCCTCCA CCGCCACCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5401312-5404821	Sequence	M271_21610 - hypothetical protein (Protein is coded in complementary strand; from 5404821 to 5401312)