Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5378078
End position: 5381539
Length: 3462

DNA sequence
>NC_022785, from 5378077 to 5381538 (3462 bp); Streptomyces rapamycinicus NRRL 5491 TCACTCCCCGGCGGCCTGTGCCGCGGCCAGCTCCGCGCTCAGATATCCGGCCAGCGCGACCGGAGTCGGG CAGTCGTAGACCACCGTCGCGGGCAGCGTCAGCCCGGTGGCGGCCGTCAGCCGGTCCCGCAGCTCGATCG CTCCGAGCGAGGCGAACCCCATCTCCAGGAACGCCCGTTCGCCGTCGACCTCCGCCGCGGAGGGGTGGCC CAGGACGGCGGCCGCCTGTCCGCGCACCACGTCGAGAAGCATCCGGTCGCGCTCCGGCCCGGACGCCTCC CACAGCTCCGACGCCACCGTCCGCGGACCGGTCTGCGCGTCGAAGGCCCCGGGGCGCCGCCCGGTCGCGA ACTCCGGCAGAAGGTCGAACAGCCGGCTGGGGCGGGCCGTGGTGAACACCTCGCCGAACCGCTCCCAGTC GACGGCGGCGGCGAGTACGGTCCCCTCCGCACCGCCGACCGTCCGGCGCAGCACGGCGATCCCCTCCGCC GGTTCCAGGGAGCCGAGCCCCAGCTCCAGCAGCCGGCCGGCGAGCGCGTCGGTACTCCACGGCCCCCAGG CCACGGAGGTGACCGGCAGCCCGCGGGCCGCGCGCCGCCCGGCCAGGGCGTCCGCGTACGCCTCCGTCAT CGCCCGTACGGCCTGGCCGACACCGCCCACCGTGCCGGTGATGGACGAGAAGACGACGAAGGCCGACAGT TCGAGGTCCTCGGCGAGCTCGTACAGATGGGTCAGACCGGCCAGCCGACCGGACACCAGCTCGTCGTACC GCGCGCCGGGCAGCCCGACGACCGGGTGGTCCTCGACCACGCCCTCGGTGTGGACGATGGCGTCCGGCGG GTACTCCGCCAGCAGACGCGCCAGCGCGTCGCGGTCGTCCAGCGGGCAGGAGGCGACAGTGACGCGTGCA CCGGCCGCGCCGAGCTCCGCCGCGAGGCCGTGCGAGTCCGAGCCGTCCCCGGTCAGGACGAGATGCCGCG CGCCGTCGGCCGCCAGCCAGCGGGCGACGTGCGCACCCACGCCCTCAATGCCGCCGGTCACCAGCACCGT ACCGGCCGGATTCCACCGCGGTCCCGCGGTGTTCTCGGTGTTCTCCGTGTTCTCGGCGCTTTGGGGGTTC TCGATGCTCTGGGGGTTCTGGGTGTTCTGGGGGTTCTGGGGGTTCTGGGTGTTCCGCTCAGTCTGGGCGT CCGCGCGCACCAGACGGCGGCCGAGGGCTCCGGCGGAGCGGATGGCCACCTGATCCTCACCGGTCGAGCT CGTGAGGACACGACACAGCCGGGTCAGCGCGCGTGCGTCGAGCCGCTCGGGGAGGTCCACGAGACCGCCC CACCGCTCGGGGTGCTCCAGCGCGGCCACCCGGCCGAGCCCCCACAGCAGCGCCGCCGAGGCCGAGGGCG CGGGCTCGGAGGGGGCGACCCCGACCGCACCATGGGTCAGCCACCACAGGGGGGCTTCAAGCCCCAGCTC GTCTGCCGCACGGACCAGTTCGACGGCCCAGGACCCGGTCCGGGGCAGGGCCACCATCCCCCGGACCGCG TCCGCCGAGGCCGCCTTCAGGGACTCGGTGAGTGAGTCACCGTCCGCCGTCACCAGCCGGACCCGCGCAC CGTGCCGCTCCAGGCCCCGGGCCACGCGTCGGACCACGTCGTCCTCGTCCGGGACGGCGTCCCCGTCCAG GGCACCGTCCCCGTCCAGGGCGGCATCCGCGCCCAGAGCCGCACCCTCGCTCAAGACGGCATCCGTGCCC CGCTGTCCGGGCACCACCAGGAGCCAGGTTCCGTCGAGCACGGGCGGGCCGTCGGCATCGACGCCGGGCA GTGGCTCCCAGCCGATCCGGTACCGCAGACCGTCGCGGAGGTCCTGGGAACGGTCCCGTGCCCGCCAAGC CGACAGCGCGGGCAGCGCGGCGTCGAGCGTGGCTCGCCCGTCCGGGTCCGTGACGCCCAGCGCCTCGGCC AGTGTCCCGAGGTCTCCGCACTCCACGGCCGCCCAGAAGCGGTCCTCCCGTTCCTGAGGTGCGGGCTGGT CCGCCCGGCCGTCCCGCATCCAGTAGCGGTCACGGGCGAACGCGTAGGTCGGCAGCTCCACCGTGCGCGC TCCCGCGAACAGCGCTGACCAGTCCACGCGGACGCCGTGCGCGGACGCCTGGGCCACCGCGCCGACCAGG GCCTCGGCCTCGGGGCGGCCGCTTCGGAGGACGGGCACCAGCAGGATGTCGTCCGCGTCCGCCACGTCGG CCAGGCAGTCCTGGGCCATCGCCGACAGCACGCCGTCCGGGCCCAGTTCGAGACAGGTCCGTACGCCCAG GTCGCGCAGCGACCGCACGCCCTCGTGGAACCGTACGGTCTCGCGGACGTGCCGTGTCCAGTAGTCCGCC GAGCACACCTCCTCGGCGGGCACCGGCACCCCGGTGAGGTTGGACACCAGGGGGATGCTCGGCGGGCGGT AGGTCAGTCCGTCCGCGACGGCGCGGAACTCGTCCAGCATGTCCTCCATGTGCGGCGAGTGGAACGCGTG GCTGACCCTCAGCCGCCGGGTGCGCCGTCCCTCGGCGACCCATTGGTCGGCGATGTCCAGTACGGCGTCG AGGTCACCGGAGACGACCGTGGCGGCCGGGCCGTTGACGGCGGCGACACTCACGAAGCTCTCGAGGCCCA CCAGCGACTCCCGCACCTCCTCCTCGCTCGCCCGGATCGCCACCATGGCGCCGCCGTCCGGCGCGGCCTG CATCAGCCGGGCACGGGCGGACACCAGCGCGCAGGCGTCGTCCAGGTCGAGGACCCCGGCGGCGTGGGCG GCGGTCAGCTCGCCCAGGGAGTGGCCCATGACGCAGTCGGGGCGGATGCCCCAGGACTCCAGCAGCCGGA GCAGGGCGGTCTCGACGGCGAACAGGGCGGGCTGGGCGTACTCCGTACGGTCCAGCAGCTCCTGGCCGGC TCCGAACACCACGTCTTTCAGGGGCAGCGGAACCCGGTCGCACACCGCGTCGAACGCCTCGGCGAACACG GGATACGTGTCGTACAGCTCCCGTCCCATGCCGACCCGTTGGGCGCCCTGGCCGGTGAAGACGAAGGCCG TCCCGCGGTCGGTGGCGGTGGCGGTCGCGGTCGCGTTCACGTGGAAGGTGTTCGCGGCGGGGAGGTCGTC GTACAGCGCCGACAGGCCCTGTAGGACGTCGGCGCGCTCGGCGGCCACCACCGCGCCCCGGAAGGGGAAG AAGCTCCGCGTGGACACCAGCGACCAGCCGACGTCGACCGGGCGCAGTCCCGGGTGGGCGATGAGGTGGT CGCGCAGCCGGAGCGCCTGCCGGCGGAGGGCCTGGGCGGAGCGCGCGGAGAGCAGAAGGGGAACGACTGG CCGGGTGCGGTCACGGTCGGGCGGTTCGGAGGTTTCGGAGATCTCGGAGATTTCCGGGGTCACGGTGGTT TCCGGGCTATCCCGGGTTTCCGGGGTGATCGC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5378078-5381539	Sequence	M271_21585 - hypothetical protein (Protein is coded in complementary strand; from 5381539 to 5378078)