Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5325476
End position: 5326834
Length: 1359

DNA sequence
>NC_022785, from 5325475 to 5326833 (1359 bp); Streptomyces rapamycinicus NRRL 5491 TTAGCCCAGGGCCGACTTCACCGCGTCCGCCAGGCGGCCCGCGACCGAGCGGGCCTGCTCGATGTCGGCG GCCTCGACCATGACCCGTACCAGCGGCTCCGTGCCCGAGGGGCGGAGCAGGACCCGGCCGGTCTCGCCCA GCTCGCGCTCGGCCTCGGCGACGGCCGCGGCCACGTCCGCGCTGGAGGCGACGCGGGACTTGTCCACGTC GGGGACGTTGACCAGCACCTGCGGCAGCCGCTCCATGACACCCGCGAGGTCGGCCAGGGTGCGGCCGGTC GCGGCGACGCGGGCCGCCAGCAGCAGGCCGGTGAGGGTGCCGTCACCCGTCGTGGCGTGGTCGAGGACGA TCACGTGCCCGGACTGCTCGCCGCCCAGGGCGTAGCCGTGGGCCTTCATCTCCTCCAGCACATAGCGGTC CCCGACCGCCGTCTGGACCAGTTCGACGCCCTCGCGCCGCATGGCCAGCTTGAAGCCGAGGTTGGACATC ACGGTGCCGACCACCGTGTCCCCGCGCAGGTTGCCCGCCTCGCGCATGGCCAGGGCCAGGACGGCGAGGA TCTGGTCGCCGTCGACCTCGCGGCCCGTGGCGTCCACCGCGAGACAGCGGTCGGCGTCCCCGTCGTGGGC GATGCCGAGGTCGGCCCGGTGCTCCACGACGGTCGCCTGGAGGCGGGCGAGGTGGGTGGAGCCGTAGCCG TCGTTGATGTTGAGCCCGTCCGGGTCGGTGCCGATCGTCACGACCTCGGCCCCGGCCCGCGCGAACGCCT CCGGGGAGACCCGGGCGGCGGCGCCGTGCGAACCGTCGATGACGATCTTGAGGCCGTCGAGCCGGTTGGG GAGGACGCCGACCAGATGGGCCACATAGGCGTCGAAGCCCTCGTCGTAGTCCCGGACCCGGCCCACCCCG GTGCCCGTCGGCCGGTCCCACGGCTCGCCGGAGGCGTGGGCGCGGTAGGTGGTCTCGATGCGGTCCTCCA GCTCGTCGGCCAGCTTGTGGCCGCCGCGGGCGAAGAACTTGATGCCGTTGTCGGGCATCGGGTTGTGGCT CGCGGAGAGCATCACCCCGAGGTCCGCGTCCAGCGCCCCGGTCAGATGCGCGACGGCCGGTGTGGGCAGC ACGCCGACCCGCAGTACGTCCACCCCGGCGCTGGCCAGCCCCGCCACCACGGCGGCCTCCAGGAACTCCC CGGACGCTCGCGGATCCCGCCCGACCACCGCCACCGGCCGATGGCCTTCGAAGGTTCCCGCCTCGGCGAG CACATGCGCCGCCGCTACCGACAGGCCGAGCGCCATCTCAGCCGTCAGATCGGCGTTCGCGACGCCGCGC ACACCATCCGTACCGAAGAGTCGTCCCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5325476-5326834	Sequence	M271_21270 - phosphoglucosamine mutase (Protein is coded in complementary strand; from 5326834 to 5325476)