Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5253194
End position: 5253745
Length: 552

DNA sequence
>NC_022785, from 5253193 to 5253744 (552 bp); Streptomyces rapamycinicus NRRL 5491 TCACGTCAACACCTGGTCTGGACTTTGCCTGATTCGCCCCATGTGTGACGGCGCATGGGAGGCCGCTCGC TTCTTGGAGAGCGACTCCTCGCCCTTCGCGCGGTCGATTTCACACCAGATCCGCTTCCCGGAGCCCTCCG GCTGCCAGCCCCAGCGGTCGGCGAGACCGTCGACCAGCTCCAGACCGCGCCCATGAGTGTCCCCCTCATC GGCGCAGCGCTGACGCGGAGCGCGGTCACTGGCGTCGGCGACCTCGACCCGGACCGTTCCCACACCGGCA GCCCGGCTCGTGAACGGCCCCGGCGTCCCGCCCCCGGCACCGCCACCGCCGCCCGTGGACTGGGCGGGGA AGAGCATCCGCAGCACGGCCGGACAGCCGGTGTGCACCACGGCGTTGGTGACGAGTTCGGAGATGAGCAG AATCAACGTCTCAGCGAGCGGCTCATCGGCTCCGATTCCGGACCCGACAAGCCGCGAACGGGCCCATCGA CGGGCACGACTCACCTCCGCCGGGTCGGGCCTGACCTCCATCTGCGCCTGAAGCACCTGCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5253194-5253745	Sequence	M271_20960 - regulatory protein (Protein is coded in complementary strand; from 5253745 to 5253194)