Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5249520
End position: 5250914
Length: 1395

DNA sequence
>NC_022785, from 5249519 to 5250913 (1395 bp); Streptomyces rapamycinicus NRRL 5491 TCATCGGCGTGATTCCTTGGGGAGGTGAAGTACGCGCTCGGCGATGATGGTGCGCTGTATCTCGTCCGAG CCGCCGTAGATGGTGTCGGCGCGGGAGAAGAGGAAGAGACGCTGGAAGGCGTCGAGTTCGTACGGCCGGC CGGCGGTCCAGCGCGCCGGGCCGAGTGCGGCCGCGGCGCCTCTCACCTCCACGGCCAGCTCGCCGAGCCG CTGATGCCAGCCGCCCCACAGCAGCTTGGCGACGCTGGGCGCGCCGGGGTCCTGCGGTCCGGAGTGCTGG GGATCGGAGTGCTGCGGGCCGGAGGCGGGTGAGCCAGGATCGGGTAAGCCGGGGGCAGGTGAGCCGGGGG CCTGGGGGTTCGCGCTCCTCGGGGGGAGGTCATGGGGGGCGGCCTTCCGCGGGGCGGGGTCCCGGGCGGG CCGGGCGGAGTTCGGGGCTGGGCTCTGCGCACTGCCCAGGGTGCGCAGGGCGTTCCAGCGCATGGCCTTG AGCTCGGCCCACTGCCGTATGAGCCGGTCTCGCAGCACGGGCTCCTCCGCGGCGCCGGTCTCCAGCGCCA GCCGCACCACCCGGCTCAGCTCATGGGCGAAGCCGATCTGCTGGACCAGGGTGGAGACCCCGCGCTCCAC CGCCAGCAGGCTCATGGCCACGCGCCAGCCGTGGCCCGCGCCGCCGACGAGGTGTTCGGCGCGGGCCACG GCCCCGTCGAAGAACACCTCGTTGAAGTCCGCGGTCCCGGTCAGCTGCCGGATCGGCCGCACCTCGATCC GTCCCGGCTGGTCCATCGGCACCAGCAGAAAGGAGAGTCCATGATGGCGCTGTGAGCCCTCCTCGGTGCG CGCCAGCACAAAGCACCAGTCGGCCTCATGGGCGAGGGAGGTCCAGATCTTCTGCCCGGTGATGCGATAG GTGCCCTCGCCCGGGTCGCGGACGGCGCGGGTCCGCAGCCCGGCGAGGTCCGAACCGGCGTCGGGTTCGC TGTAGCCCTGGCACCACACCTCCTCGCCACGGGCGATGGGCGGCAGAAAGCGGGTGCGCTGGGCGGAGTC GCCGTGGGCGAGCAGGGTGGGCCCGAGGAGGTTCTCGCCGATATGGCCCAGTCGGCCGGGCGCCCCGGCG CGGGCGTACTCCTCGGCCCAGACGACCTGTTGGGTCAGCGTCGCGGCGCGGTTGCCGTACGCCCCGTCCG TACGGTCCCAGCCCAGGCCGATCCAGCCGCCCGTGCCGAGCTCGCGCTCCCAGGCGCGGCGGACGGCCAT GTCGCCGTGCGCCTCGCCGGGGTGGCCGCCGGTCCGCTGCTTGAGGGCGGCGAACGGTCCGGTGAAGTGC TCGGCCAGCCAGTCGCGCGCCTCGGCGCGGAAGCGCTCGTCCTCCGGCCCGAAAGCGAAGTCCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5248057-5249523	Sequence	M271_20940 - aldehyde dehydrogenase (Protein is coded in complementary strand; from 5249523 to 5248057)
ORF.. 5249520-5250914	Sequence	M271_20945 - acyl-CoA dehydrogenase (Protein is coded in complementary strand; from 5250914 to 5249520)