Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5131635
End position: 5133488
Length: 1854

DNA sequence
>NC_022785, from 5131634 to 5133487 (1854 bp); Streptomyces rapamycinicus NRRL 5491 TCATACCACCTGTGCCTTCACCAGACGGACCGGCTCCGGCCCCTGACTGTGCACGTGGTAGGCGGTGACG GACGCCGGGACTGCGAGAGTTTCGGCATAGTGTACGGAGTGCCGGTGCCCGGCGGCGGTGGTGATGACCG CGCCCTGCCCGTCCACGACGGTCAGCACGTGGAAGCGGCCGTCGGTCCGCTGCTCGGCGGCCCGGTCCGG TTCGACGGCGATCCGGTGCACCTGGAAGAACATCGCCGGCAGCGCGCCCAGCACCTCCTCCCGCCAGCCG TCACCCGCGCGCAGCGGCCGGGGCCGCTGGATCAGCTCGCGGGCGACCGCTCCACCCGACCGCTCCGGGT CGAGGTTGCGGAAGGCGTGCTCCACGTGCACGGCACGCTGCCGGTCCGCCGCGTCCCGCCGCAGCCAGTC GTAGAACCGCAGTGAGTACAGATAGGGGGTGGCGCTGACTTCGAGTACGACATTGCCCTCGCCGCTGCCG TGCGGCGTACCGGCCGGGACCAGGTAGAGCTGGCCGGCCTCGGCGGGGAACACCTGCACATACCGCTCGA TGTCGAACGGTGTGCCGTGCGCGTCGGCGGCGTGCGCGCTGTGGTGGAACGCCTCGACGTCGGCGTCCTC GCGCAGTCCGAGGAAGACCTTGCCGTGCTCCCCGGTGTGCATCAGGTAGTAGGTCTCGTGCTGGGTGTAC GGCCAGCCGAAGACGTCGCGCATGTCGTCGGGCCTGGGATGGCAGTGGACGGACAGATTGCCGCCGCGGA CGGTGTCGAGGTAGTCGAAGCGGACCGGGAACGACGTGCCGAACATCTCGTGCACGGCCGGGCCGAGCAC GTCCACCGGGGAGAGCGCGACGACGAGCTGGAACGGCACCTCCACACATCTGGAGGCGTCGTCGCCGAGC AGGACGCCGCTCTCCGGCGCGATCAACTCGTAGCCGAGGGCCGTGTTCTCCGCGTCCGGGTTGTGACCGA GCGTCTCCTGTGCCCAGTGCCCGCCCCACGGCGTCGAGTTGAAGGTCGGCCGGGTGCGGAAGGGGCGGGT GGCGAGCGACCGGCAGGTCTCGCGCAGCGCCTGCCCACCGATCGACGTCGGGGTTACGGTATCGGTAACG TCGATCCATCGCGCGATCCGCTCCGCCATGTGGTCGCGGTGGCGGTCGAGCAGCGGCCAGTCGATGTAGA GCAGCCGCCGCAGGGTGGGCGGCTCGCCCTCGGGCGCCGCGAGGTTACGGCCCCGGCCGTGCTTGACCGC CGCCTCCGCGTACCGCTTCGGCAGATCGGCCCACCACAGGATGTCGGACTCCGCGAGCGCGGCGCCGGGC CCGAGCAGGACGCGCAGCGCGCCGCCCTCGGCCGGGCCGGACTCGCTTGCGCCGCCCCCGGCCGGGCCGT CCTCGCTCGGGTCGGACCCGCCCGCGCCAGCCCCGCCCGCACCGACCTCGCCCGCACCGGATTCGTCCAG GTCGGCCAGCGCCGCCAGGGCCGCCAGGGCCGCCGGGTCCATCAGGTCGGAGAGGTCGCCGCCCGCCAGC CGCGCGAAGTCGGGGTCGTCCCGTAGCTCGTCGGAGTCGGTGAGCCGCCGCACGGTCTCCCAGTCGCGCA CCGCCGTACGGACGTCCGCGAACTCCACCCGGGCGCCCCGGGCACCGAACGCCGCGCCGAGCCCGGCGGC GACGGCGGCCCAGTCCAGTACCGCGGGGCCGTCCACGGCGAGGACCCGGGTCCCCTTCGGGAGCGTCCCG GCCGCGTCGGCCCAGCCGACGGCGACCGCGTCGGCGAGGACCGGATAGCACGGCGTCGGGTCGTACTGCG GCGCCTTCACCTTGACCTGCGGACTCTCGCCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5130616-5131638	Sequence	M271_20475 - hypothetical protein (Protein is coded in complementary strand; from 5131638 to 5130616)
ORF.. 5131635-5133488	Sequence	M271_20480 - ROK family transcriptional regulator (Protein is coded in complementary strand; from 5133488 to 5131635)