Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5113372
End position: 5113971
Length: 600

DNA sequence
>NC_022785, from 5113371 to 5113970 (600 bp); Streptomyces rapamycinicus NRRL 5491 TCAGGCCGATCGCGCGGGCCGCGGGCCGGCCAGGGCCGTGAGCCGATTGCCCGAGCGGCGGCCCGCCCAC AGCACGACCAGGAAGGCCAGAGCGAGAAGGGGGTAGCCCATCGCGATCTTGGTGAAGGCGAGCCAGCCGG TGGCGTCCTTTTCGTAGAGCCACTGCTGTACGGCGACCCGGGCGGCGAAGACGGCCACCAGGGTGAGGGT GGCGATGTCGTAGTAGTGGCGCGAGGGCTTGTCCTTGAGCCAGGCGGTGCCGGTGCCGTTGAGCGAGCCC CAGACGATTCCGGCCAGCGGGCGGCGTACGAGGACCGAGAGCAGGAAGAGCACGCCGAGTGCGAGGTTGC CCCAGATGCCCGTCAGGAAGAAGCCCTTGGCCGATCCCGTCTTCCAGGCGACGAACGAGGCGACGGCGAC GCCGAACAGGCCGGAGAGCGCGGGCTGAATCGACTCCTTGCGCACCAGGCGCAGCGCGGAGATGGCCAGG GAGACGCCGACGGCGGTGCAGATGGCGGCGGTCAGCCCGTAGGAGGCGTTGGCGAGCACGAAGGCCGCGA CCGGCAGCATCATGTAGACCAGGCCTGTGGTGCCGCCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5113372-5113971	Sequence	M271_20365 - hypothetical protein (Protein is coded in complementary strand; from 5113971 to 5113372)