Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 5055154
End position: 5056461
Length: 1308

DNA sequence
>NC_022785, from 5055153 to 5056460 (1308 bp); Streptomyces rapamycinicus NRRL 5491 GTGTCCACCCCGCACATATACGACTTCATCGCGGTCGGCCTCGGCCCCTACAACCTGGGCCTCGCCTGCC TCACCGAGCCGATCGGCGAACTCGACGGACTGTTCCTGGAGAGCAAGCCCGACTTCGACTGGCATCCGGG CATGCTGCTGGACGGCGTCACCCTCCAGACCCCGTTCATGGCCGACCTGGTGACCCTGGCCGACCCCACC TCCCCGTACTCCTTCCTCAGCTACCTCAAGGAATCGGGACGGCTGTACTCCTTCTACATCCGCGAGATCT TCTATCCGCTGCGCGCCGAGTACAACGACTACTGCCGCTGGGCCGCCGCGAAGCTCCCGTCCGTCCGCTT CGGCCACCAGGTGACGAGCGTCGAGTACGACGAGGCCGACGGCCTCTACACGGTGCGCGCCCTGCGTCCC GCGAGCGGCGAGACCACCACCCACCGCACCCGCCACCTGGTACTGGGCACCGGCACCCCGCCGTATCTGC CCGAGGTGTGCCGGGACCTGGGCGGCGATCTGCTGCACAACACGGACTACCTGGAGAACAAGCGGGCGCT GCAGTCCAAGAAGTCCATCACCGTCGTCGGCAGCGGCCAGAGCGCCGCCGAGATCTACCAGGACCTGCTC GCCGACATCGACACTCACGGCTACCGGCTGAACTGGGTGACCCGCTCCCCGCGCTTCTTCCCGCTGGAGT ACACCAAACTCACCCTGGAGATGACCTCGCCCGAGTACGTGGACTACTTCCACGCGCTCCCCGAGGACAC CCGCTACCGGCTGGAGGGCGAGCAGAAGGGCCTGTTCAAGGGGATCGACGCCGACCTCATCAACGGCATC TTCGACCTCCTGTACCAGAAGAGCGTCAGCGGCCCGGTGCCCACCCGGCTGCTGACCAACACCGCGCTCA CCGACGCGGCGTACGACGAGCAGTCCGCCACGTACACCCTCGGGCTGCGCCATACGGAGCAGGAGCGGGA CTTCTCGCTCACCACCCAGGGGCTCGTCCTGGCCACCGGCTACCGCTACCAGGTGCCCGCCTTCCTCGAC CCGGTGCGCGACCGCATCCGCTGGGACCGCCACGGCCGCTTCGACGTCGCCCGCAACTACAGCATCGACA CCACCGGGCGCGGCATCTTCCTCCAGAACGGCGCCACCCACGCCCACAGCCTGACCTCCCCGGACCTGGG CATGGGCCCGTACCGCAACGCGTGGATCATCCGTGAGCTGCTGGGCCGGGAGCACTATCCGGTGGAGAAG TCCATCGCCTTCCAGGAGTTCGGCGCACCGGAAGGAGCCGTGTCATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 5055154-5056461	Sequence	M271_20110 - Alcaligin biosynthesis enzyme
ORF... 5056458-5057201	Sequence	M271_20115 - hypothetical protein