Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 4981539
End position: 4982015
Length: 477

DNA sequence
>NC_022785, from 4981538 to 4982014 (477 bp); Streptomyces rapamycinicus NRRL 5491 TCAGTCGCTGTGGTGCTGAAAGGGATGCAGCAGATTCGGAAGGTGCCAATGGTGCCCACGGCGTCGCCCG GGGTCCTCGTTCTCCGGTGCGCGGTCCGGCGCGGCGTCCGCCTCGGCCTCCGGCGCGCCGGTGCCGTCCT TCCGGTCCCGGGTGGTGTGCGGCCTCCCGCGCCGCGCACTCTCCAGGACGGAGGAGACCTGACCCGAGCG CATGATCCGCACCAGATGCGCACCGCAGTTGACGCAGTCGGGGCTCGTCAGCGGGGACGGGACGCGCCGG CCGCCGGTGAAGTAGACGACGAAGGCCCGGCCCTCACCGTCCACGTGGTGTTCGATGTCGTACGACCGCT CCCAGCCGTGCCCGCACTTCATGCAGGCGAAGGCGTACGCCTCGTGGGCCACCTCGCCGGACTGATCGGA GGGGGTATGGGCGGCTATCGGATGAGCACCGCTCGAGGTTCCCGCTATATCGCTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4981539-4982015	Sequence	M271_19755 - hypothetical protein (Protein is coded in complementary strand; from 4982015 to 4981539)