Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 4971471
End position: 4972199
Length: 729

DNA sequence
>NC_022785, from 4971470 to 4972198 (729 bp); Streptomyces rapamycinicus NRRL 5491 CTACGGCGCCGACGCGGGCGGCTGCGGCGGGGCGGGGGGCTGCAGCGGGGGCGGCGCGGTCCGGCGCGTC GCCGCGCGGTCCAGCAGCCCCGTACGCGCCGCCAGGGCCGCCGCCTCCAGCCGCGAGCCCACCCCGAGCT TCATCAGCACCCGCTGCACATGGGTGCGGGCCGTGCTGGGGGCGATCCCCATGCCCGCCGCGATCAGCCG GGTGTCCTCGCCCTCGGCGACCCGCACCAGCACCTCCACCTCGCGCGGGGTCAGCATCCGCAGCAGCCGG GCGCCCTCGTCGTCGGGCTGGGCCGCGGGGTTGAGCAGCTCCGCGAACGCCCCCTGGAGCAGCTGGGGCG CCACGGCCGCCTCGCCCGCGCGCGCCTTCATCATGGCGCGTTCGACGCCCTCGATCCGCTCGTCGTGACG CACATAGCCGGACGCCCCGGCGGCGAAGGCGGCGGCTATGCCGCGCGGGCTCGGCACCGGACCGAGCACC ACGACCGCGACCTGTGGCCGCTCCTTCTTGATCCGCACCACCGGGTCGAACACCCCCGGTTCGGCGGGTG CCGCCGTGCCCAGCAGGCACACCTCAGGTGCCCGGCTCACCACCAGGTCCGCCGCGCCCGCGCTCGGCGC CGCCGCGGCGAGCACCCGGTGCCCGCGCAGCTTCAGCGCCGAGGCGAGCGCCTCCGCGAGCAGACGGTGA TCGTCGACCACCATGAGTCGCACGCCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4971471-4972199	Sequence	M271_19720 - transcriptional regulator (Protein is coded in complementary strand; from 4972199 to 4971471)