Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 4858528
End position: 4859148
Length: 621

DNA sequence
>NC_022785, from 4858527 to 4859147 (621 bp); Streptomyces rapamycinicus NRRL 5491 GTGGTGCGAGAGCGGCGGGCAGCACGGGATGGCCGCCGGGCCCGGGAGTTCGAGGCATTCGTCGCGGGCG CGGGTGGCCGACTGCTGCATGCGGCCACGCTGCTCACGGGGGAGCCGACGGGGGACACCGAAGCCGCCGA GCGCCTGCTGACCGCCGCGCTCGCGCGCACGTACGCCCGCTGGGACCGGCTGCGCGGCGAGGATCCGTAC GACCACACCCGCCGGACGCTGGCCGCCCTCTTCGCCCACCGGGGCCACCGCTACCGCCGCCCGCGCGGCG GCACCCTGGCCCGGCTCACCCCGCAGGAGCGGCTGGTGCTGGTGCTGCGGCTGTACGAGGGGGTCGCGGA GGAGCAGACGGCGGCGGCGCTCGGGCTCCCGGTCGAGCGGGTCCGGGCGATCTGCACCCGCGCGGTCACG GCGATGCGCAGCGCGGCGGCCCGGCGCGGCACGACTGGAACGCGACCGTCCGCCGGAACGGTACCCTCCG GCGGAACGCCATCGTCCGCCGGCCCCACCGGCCCCACCAGCCCCACCGGCAGCCGGGCGGCCACCCGCGC GACGGAGGCCGGAGCCGACGCCGGGGCAGGAGCCGAAGCGGGGGCCGGGGCCGCCCCATGA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4858528-4859148	Sequence	M271_19170 - hypothetical protein
ORF... 4859145-4859399	Sequence	M271_19175 - hypothetical protein