Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 4510655
End position: 4511629
Length: 975

DNA sequence
>NC_022785, from 4510654 to 4511628 (975 bp); Streptomyces rapamycinicus NRRL 5491 TCACAGCTCGTCCTCCTCTTGCCTGAGCACGATCCGGAGGTAGTAGGAGGTCAGCGCCAGCAGGACCGCG ATGGTCAGCAGCGAGATCGCGGCGCCCATGCCGTAGTGCTGGTTGCCGACGCCCTCGATGAAGGCGTAGA CGGGCAGGGTCTCGGTGAGCCGGTCGGGGCCGCCCTCGTTGATCGCGAAGACCTGGGTGAACGCCTTGAA GACCCAGATGACTTCGAGGAACGTCGTGGCCAGCAGGAACGGCTTGAGGAACGGGAAGGTCACCGAGGTG AAGCTCTTCCAGCCGCCGGCGCCGTCGAGCGCGGCCGCCTCGTAGAGCTCGCCGGGGATGGTGGTGGTCG CGGCGTAGAGGTTGATCGCCACGAAGGGGATCGACCCCCAGACGATCAGCACGGTGATGACGAAGAAGGT GGAGAACTGGCCGCCGGTCCAGTTGTAGTGCGCCATGGAGTGCCAGCCGAGCGCGTCCAGCACCCAGTTG ACCACCCCGAAGCGGGTGGCGAAGAGCCACTGGAAGACGGTGGTGGCGGCGACGGTCGGCATGGCCCAGG TCAGCACGAGTCCGATCGAGAGCAGCAGCCGCATCTTGCTGCCGAGCCGGGCCAGCAGCAGTCCGATCAG GGTGCCCAGCAGCATGATCAGCACCACGTTGACCGCGGTGAAGATCACCGACCGCAGGGTGACCCGCCAG AAGTCCTCGCTGGTCAGCGCCTCGCGGTAGTTGTCGACCCCGTTCCACTCGGTGAGATGCTGGATCAGCT GCCGCATATTGAGGTTCTGGAACGACAGCAGGCCGTTCTTGACCAGCGGCCAGCCCAGCAGCAGCGCGGT CGCCGCCAGCGCGGGCAGCAGCAGGAGGTACGGGGCTGCGCCATTCAGCCGTGCGCGCCCGGCGGGACCC GGGGCCGGGCGGCGGGGCCCCGGCGGCGCCCNNNNNNNNNNNNNNNNNNNNNNNNNCGGGCTCAA

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4509828-4510658	Sequence	M271_17695 - sugar ABC transporter permease (Protein is coded in complementary strand; from 4510658 to 4509828)
ORF.. 4510655-4511629	Sequence	M271_17700 - sugar ABC transporter permease (Protein is coded in complementary strand; from 4511629 to 4510655)
ORF... 4511626-4512858	Sequence	M271_17705 - sugar transporter (Protein is coded in complementary strand; from 4512858 to 4511626)