Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 4251469
End position: 4253163
Length: 1695

DNA sequence
>NC_022785, from 4251468 to 4253162 (1695 bp); Streptomyces rapamycinicus NRRL 5491 TCACAGCGCCTTGGTGAGCTGGCCGACGTCGTTGAGGGAGCTTCCGGCGACGAGTCCCGCGCCGACCAGC GACAGATCGTTCTCGGCCTGCTCGTCGGACTCCTTGCGCGCCCTCGCGTCGGCGATCCCGCGCCGCCGCC ACATGGTGTAGAGCTGACGGATCACCAGGGCACCGAGGACCATCCATCCGGCCTGGGGCGTGGCCACCAG AAGGCCGGTGGCCAGCAGGACGCCCATCTGCCGGGAGGGTCCGCCGATCAGCTGGACCAGCGCGCCGGGG ATCGCCCACAGCAGCATGGTGGTCATGGTGCCGGGCTCGAGGCCGGTCTTGATGGTGGTGACGTAGACCT CGGCGACCGGCGGCACCTTGCCCTCGGAGAAGAAGGCCTTCCAGGCGACGGCGACGACGACCAGCGCCAC ACCGAAGCCGACGAGCTGGGCGAGGTACTGCTGGCGCCGCCCCTCGATCTCGAAGGGCCGCCAGGGCGCG GCGTCCCGGCGCAGCAGCCAGCCCGCCTTGAAGTCGTAGCCGAGGTCGGCGAAGGCGGGGCCGGTGGAGG CCGTGTAGCCGACCAGCAGGGCGAGCGGGACGGTCGGGATGTGCAGCACCAGACCGATGATCAGGAAGAT CAGGGTGACGGCGAAGGCCGGGAACCAGCCGGAGTGCATCGCCGCCAGCCCCACGATCAGCTGGTGGACG ATGGCGGCGACACCGGCCAGCAGCACCCAGCCGACCAGGGCGGGCACGGAGAGCCCGCCGACCACGCCGC CCGCGATCGCGACCACGACCGCGCCGCAGACGAAGAGGCCCATGCCCTCCAGCAGTCCCCGCCGCAGCTG GGGGGCCTGGACCGTACGGCCACCGTCGGCGTCCGGGCCACCGTCGGCATCCGGGCCGCCCTCGGCGCTC TCCGCCCGATCGGCCCCGTCGCCCCCGCCCTTCCGCGTCCGCATCAGCAGCAGCGCCTGGGTCAGGGCGA CCAGCCCCGCGCCGATCATCAGCCCGTGCGGCACATGGTTCCCGCCCAGGTCGAAGCCGAAGTCGGGGGC GTACTGCCCCACGGTCAGCCCGATGCCGAACATGGCGAGCGCCCAGATGTTGCCCAGGAAGCCCACACCC GCGGCGCTGGTCGGCAGCTTGAACAGCGCGCCGACGAAGCCGACCACGGCGCCCACGCCCAGGATCGCGG CCTTGCGCCCGCCGGTGTCCCCGGCCTTGATCGTCTCGGCCGCCGCGATACCGGCGGGCCAGGAGCCGGT GGCGGGCAGCAGCTTGGAGTCGAACACCCGGTACAGCACCCAGCTGTCGACCGCCAGGCCGATGACGGCC CCGCCGAGCATGGGCCACACCAGGTCGTTACGCCCGAGGGCGAACGGCACCGCGATGGGGGTGAGCAGGG AGTTCGCCGCGGCGAAGGTGGAGCCGGAGATCGCCGACTGCACCAGGTTCTGCCGGTGCTTCGAGCGCAT CCGGGTCAGCACGCCCACCGGGACGCGGCCGATGAGCATCGCCACCAGGGCGCCGATCACCGCCGTATTG GGCGACACCCCGAGCGTGGTGATCAGCACGATGCCGATCAGGGCGCCGAGGACCGACAGCACGATGGTCA GGACGAGGGTGACCGGTTCGAAGGCTCTGGGGTGCGACCGCGGTGGGGCGTCCGCGGCCGCGGTGACCTG GGGGTTCGGGTTCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4251469-4253163	Sequence	M271_16545 - membrane protein (Protein is coded in complementary strand; from 4253163 to 4251469)