Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 4164437
End position: 4167352
Length: 2916

DNA sequence
>NC_022785, from 4164436 to 4167351 (2916 bp); Streptomyces rapamycinicus NRRL 5491 TCAAGTGGGTGGGACGGGCGTCTCAGCATTTGAGCGGGTCGGCGGATGGGTGTGCGGCTCCGTACGGCGC GGGCCGTGGGAGCCGCCGTGCCCGCCATGAGCGCCGTGCCGGATCGGCAGGGCGCCGCCGACCAGCAGCG GGGGCTCCGGGCGCGGCACCGGATAGCCGCCGCGCAGCAGTGCCCGGCGCAGCCGGTGCTCGTCCAGCGG CCCGCTGAAGGCCGCCCCCTGGGCGTGGGTGCATCCCATACCGCGCAGGGTGACAATCTGCTCGGGCAAC TCCACCCCCTCTGCGATGGACTGCATTCCGAGATCGCCGGCGATCCTCAGCAGCCCTGCGGTGATCTTGT GCAGCCGTGAGGACTCGACCACTCCCTCGACCAGTCCGGGGTCCAGTTTCAGCACATCGATGGGGAGCCT GCGGAGCGCCCTGATCGCGGCATAGCCGCTGCCGAAGCCGTCGAGGGCGATCCGCACCCCCAGACGGCGC AGCGCGCCGAGGCGGTGCTCGAGCTCGTCCAGCGGCACCCGTGGATCGCTGTCGGTCAGCTCCAGGATCA GCGCGCCGGACGGCAGCCCATGGCGGGCCAGCAGGGTCTCCACGACCTTGGGCGGCATCGACTTGTCCAC CAGCCGGCTCGCGGGGAGCCGTACGCAGACCGGGACCGGATGGCCGTCGCGGCGCCGCTGGGCGGCCTGC TCCACGGCCGCCTCCAGCATCCAGCGGGACAGCTCGGCGGTACGGGCGGTGTCCTCGCCGCTCCCCCGGC CGTTGTCGGCGACCCGGAGGAACTCGGCGGGGGTGAACAGGATGCCCTGGGCCGAGCGCCAGCGGGCCTG CGCCGCGACCGCGGTGATCCGGCCGCTGGTGAGCTCCACGACGGGCTGGTGCAGCAGTACGAACTCCCCG TCGTGCAGCGCGGCACGCAGTCTGGTGGCGACCTCGGCGCGCCGCGCCACCTCGGCCTGCATCTGCGGTG CGTACAGCTCCACGCGGTTCTTGCCCGCGGCCTTGGCGCGGTACATCGCCAGGTCGGCGTTGCGCATCAG GGCGCCGGGGCTGGTGCCGGGCTCGGCGAAGGCGACGCCGATGCTGGCGGCCACGCGGACCTCGGTGCCG CCCTCGACCCGGTAGGGCTGGGAGAGCCGGAGCCGCAGCCGGTCGGCGATCTCCATGATGCGCTGCTCGC GGGTGCCCGTGTCGCCCTCGCCGTCACCGAGGATGAGGGCGGCGAACTCGTCGCCCCCGAGCCGGGCCGC GCAGTCGCCGGACCCGGACCGCACGGACTCCTGGAGCCGGCGGGCGGCCTGGACCAGCAGCTCGTCCCCG GCGTGGTGGCCGACGGTGTCGTTGACCGCCTTGAAGCCGTCGAGGTCGATGAAGAGCACGGCGGTGCCGG CGTCCGTACGGCGGCGGCCGGCGACGGCGTGCGCGACCCGCCGGGTGAACAGGGCGCGGTTGGGCAGATC GGTGAGCGGGTCGTGCTCGGCGTTGTGCTGGAGCTGGGCCTGGAGCCGGACCCGCTCGGTGACATCGCGG CTGTTGAAGATCAGCCCGCCGTGGTGGCGGTTGATCGTGGACTCCACGTTGAGCCAGTCGCCGGAGCCGG AGCGGAAGCGGCATTCGATCCGCGTGGTGGGCTCCTCGCCCGGTGCGGCGGCCAGGAACCGGCGGACCTC GTGGACCACCGATCCGAGGTCCTCGGGGTGGATGAGGGAGGCCAGCTCGGAGCCGATCATCTCGTCCGCG TCGCGGCCGTAGACCCCGGAGGCGGCCGGGCTGACGTAGCGCAGGATTCCGGTGGGTGCGGCGATCATGA TCACATCGCTGGAGCTCTGCACCAGGGAGCGGAAGTGGTTCTCCTTCTGGGCGAGTTCCTGGGTGAGCCT GATGTTGTCCATCAGCATGATCGCCTGTCGGACGACGAGTGCGAGCACGACCGCGCAGGCGGTGAAGAGC ACCACCCCGTCGATCGGCCGGCCGTCCATCACGTTGAACAGGACCCCCAAGGTGCATACGGCTGCGGCGA GATAGGGCGCGAGTGCGGCGAGCGAGCCCGCGATGGGGACGCTCTGCTGCGGGGAGCGGCGCGCAGACGG CGGGTTCTGGGCGGCCTCGCGGCGGGCCACCCATGGTGCGTACGCCAGCAGCGCGGAACCGGCGAACCAC CCGGCGTCGAGTATCTGCCCGGAGCGGTAGTGCTCCCGCAGCAGCGGCGAGGTGAACAGCGCGTCGCACA GCACGGTGAGCGCGAAGGCGGCGATCGCGGTGTTGATCGCGGAGCGGTTGCCCGAGGAGCGCCGGAAGTG GAGCACGATCACCATGCTCACCAGCACGATGTCCAGCAGCGGATAGGCGAGCGACAGGGCGCCGCGGGCC ACGGAGGCGCCGTCCGCGCCGAACTCCCCGGTGTGGGCGAGCGCGAGGCTCCAGGAGAGCGTGACCAGCG ATCCGGCGATCAGCCAGGCGTCCAGCCCCAGACAGATCCACCGGGCCCGGCTCGTGGGGCGCTTGGCGAG CACCAGCATCCCTACGATGGCGGGCGGGGCGAAGAGCAGAAAGCAGAAGTCGGCGACCGAGGGGGTCGGC ACGGAGCGGCCGAGCACCACCTCGTACCAGCCCCATACGGCGTTCCCCAGCCCGGCCATGGCCGAGGAGG CGGCGAACAGCAGCCAGGCCGGGCGCAGCCGCCCGGCGTGCCGGCGGCCGTGCCACAGACAGGAGGCCGT GGCGGCGAACGCGGCGGCGCTCAGTCCGAAGTCACCCATGACGAGGGCCAGTTGGGACGATCCCCAGCCG ACGATCGCCCCCACCGCGTAACCGCCGCAGACCAGGGCGAGGACCACTTGCGGCAACAGCCCGGCCCCGC CGCCGGAGACCGACGGCCGGGCGAGCACCGCTCCCGTGGCGCTCAC

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4164437-4167352	Sequence	M271_16190 - integral membrane phosphodiesterase (Protein is coded in complementary strand; from 4167352 to 4164437)