Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 4146887
End position: 4148518
Length: 1632

DNA sequence
>NC_022785, from 4146886 to 4148517 (1632 bp); Streptomyces rapamycinicus NRRL 5491 TCAGCCCATGTGCGGGTAGCGGTAGTCGGTCGGCGGGACCAGGGTCTCCTTGATAGAGCGGGTGGAGGTC CAGCGCATCAGGTTCTGCTTGGCGCCCGCCTTGTCGTTGGTGCCGGACGCGCGGCCACCACCGAAGGGCT GCTGGCCGACGACGGCGCCGGTCGGCTTGTCGTTGATGTAGAAGTTGCCGGCCGCGAAGCGCAGCGCATC ACAGGCGGCGGCCGCCGCCGCGCGGTCCTGGGCGATGACGGCGCCGGTCAGCCCGTACGGGGCGGCCGAC TCCATCTGCCGCAGCATCGCGTCGTACTCCTCGTCCTCGTAGACGTGCACCCCGAGGATCGGGCCGAAGT ACTCGTCCTTGAAGATCTCGTTCTCCGGGTCGGTGGAGACCAGCACGGTCGGGCGGACGAACCAGCCCTC GCTGTCGTCGTAGGAGCCGCCCGCGACGACCTCGACCGTCGGATCGGACTTCGCCCGGTCGATCGCCGCC TTGTTCTTGGCGAAGGCGCGCTCGTCGATGACCGCGCCCATGAAGTTGTCGAGGTCGGCCACATCGCCCA TGGTCAGCCCGTTGACCTCGGTGGCGAACTCCTCCTTGAGGCCGTTCTCCCACAGGGAGCGCGGCACATA GGCGCGGGAGGCCGCGGAGCACTTCTGGCCCTGGAACTCGAAGGCGCCGCGGGTCATCGCCGTCTTCAGC ACCGCCGGGTCGGCGGACGGGTGGGCGACGATGAAGTCCTTGCCGCCGGTCTCCCCGACCAGCCGCGGAT AGGTCTTGTAGTTCTCGATGTTGTTGCCGACCGTCTTCCACAGGTACTGGAAGGTCCGGGTCGAACCGGT GAAGTGGATGCCCGCGAGGTCCGGGTGGGCCAGCGCCACCTCGGAGACCTCCTTGCCGTCGCCCGTGACC AGGTTGATCACACCCTTGGGCAGCCCGGCCTCCTCCAGCACCTCCATCAGCAGCACGGCGGCGTGCGTCT GGGTCGGGGACGGCTTCCAGACCACCACATTGCCCATCAGGGCGGGGGCGGTCGGGAGGTTGCCCGCGAT GGCGGAGAAGTTGAAGGGGGTGATCGCGTAGACGAACCCCTCGAGCGGCCGGTGGTCGGTGCGGTTCCAG ACCCCGGGCGAGTTGGTCACCGGCTGCTCGGCCAGGACCTGACGGGCGAAGTGGACGTTGAAGCGCCAGA AGTCGATCAGCTCACAGGGGGTGTCGATCTCCGCCTGCTGCGCGGTCTTCGACTGGCCCAGCATGGTGGA CGCGGCGAGCGTCTCCCGCCAGGGGCCGGAGAGCAGATCGGCGGCCTTGAGGATGACCGCGGCGCGGTCG TCGAAGCTGAGCGCGCGCCACGCCGGGGCCGCGGCCAGGGCCGCGTCGATCGCGTCCTGCGCGTCCGCCT GAGTGGCGTTGGCGTAGGTGCCCAGGCGGGCGGAGTGCTTGTGCGGCTGGACGACGTCGAAGCGCTCCCC CGCTCCCATCCGCTTCTGGCCGCCGATGGTCATCGGCAGGTCGATGGGGTTCTCGCCGAGCTCCTTGAGC TTGGTCTCGAGGCGGGCGCGCTCCGGGGTGCCGGGGGCGTAGGTGTGCACCGGCTCGTTCACCGGCACGG GGACCTGGGTGACGGCGTCCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4146887-4148518	Sequence	M271_16130 - 1-pyrroline-5-carboxylate dehydrogenase (Protein is coded in complementary strand; from 4148518 to 4146887)