DNA sequence >NC_022785, from 4144850 to 4144949 (100 bp); Streptomyces rapamycinicus NRRL 5491 CGGCGNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNN NNNNNNNNNNNNNNNNNNNNNNNNNNNNNN Translate to protein Restriction digest BLAST Design primers with primer3