Sequence retrieval

Genome: Streptomyces rapamycinicus NRRL 5491
Start position: 4050840
End position: 4052570
Length: 1731

DNA sequence
>NC_022785, from 4050839 to 4052569 (1731 bp); Streptomyces rapamycinicus NRRL 5491 TCAGAACTCTCCGGAACGGTCGGTGCGGATGCGCTCGAGCGCCAGCAGCGATCCGGCGCAGACCAGCATC AGGATCGTGCTCAGGGCCATCGCCTGTCCGTAGGTGAGCTGTCCGGCCCGGCCCAGGAAGCGGGCGATGG CCACCGGGAGGGTCGGATGGTCGGGGCGCGCGATGAAGACGGTCGCCCCGAACTCGCCCAGGGAGACGGC GAACGCGAAGCCCGCCGCGACCGCCACGGCCCGTCCGACCAGCGGCAGGTCCACCTCCCGCCAGACTCGC CACGGGGACGCCCCGAGCACGGCCGCCGCCTCCCGGAGCCGGTGGTCCACCGCCCGCAGCACCGGCAGCA TGGTCCGTACGACGAAGGGCACGCCCACCAGGGCCTGGGCGAGCGGCACCAGGACCCAGGAGGCGCGCAG ATCCAGCGGGGGCTCGTCCAGGGCGATCAGGAAGCCGAATCCCACGGTGACGGCCGAGGTGCCCAGCGGC AGCATCAGCAGCGCGTCGAAGCCCCGCACCAGCCTGCCCGCCTTACGGGTGAGCGCCGCGGCGGCGAGAC CGCCCACGACGAGGGCGAAGACGGTGGCGACCGCCGCGTACCACAGGGAGTTCCCGACGGCCTCCATGGG AGCCACGAAGAAGGTGCCCCCACTGTCGGCGGCCGACCGCAGCGCCTCGTAGTAGGTGAACCCGTAGCCG TCCGGCCCGTTCAGCGAGCGCTCCACCAGCACCGCGAGCGGTGTCACGATCAGCAGCGCGACCATGGCCA GCACGCCCCACAGCAGCGCCCACTGGGCCGGGCCCTTCGGGCGGCGCGCGGTGCGGCCCGGGTCCACGAG CCGCAGGGCGCTCTCGCGACGCCTTACGGTCCACGCGTGCAGGGCCAACACGGCCGCGACCGCGGCGAAC TGGACGAGGGTCAGCACCGCGGCCGTGGGGAGGTCGAGCAGTTGCGCGGTCTCCCGGTAGATCGCCACCT CCAGGGTGGCGTAGCGGGGGCCGCCCAGGATCTGGATCACGCCGAAGGAGGTGAAGGTGAACAGGAACAC CATCAGCGCGGCGGCGGCCACGGCCGGGGCCAGGGCGGGCAGCGTCACCCGGCGCCAGGCCGACGGCCGG CTCGCGCCGAGCACCCGCGCGGCCTCCTCCTGACGCGGGTCGAGCTGGGCCCACAGCCCGCCCACGGTCC TGACGACGACCGCGTAGTTGAAGAAGACATGGGCCAGGAGGATCGCCCAGACGGAGGTGTCCAGCCGCAC CCCCCACAGCTCGTCCAGCAGCCCGCCCCGGCCGAGCAAGGCCAGGAAGGCAGATCCGGCGACGACGGTC GGCAGGACGAACGGCACGGTCACCACGGCCCGCAGCAGCCCCTTGCCGGGGAAGTCGAAGCGGGCGAAGA CATACGCGCCGGGCAGCGCCACCGCGAGCGTGAGGGCGGTGGACGCGACCGCCTGCCAGCAGGTGAACCA CAGGACGTCCAGCGTCGCCGGATCGGTCACCACTTCGGCGATCCGGCCGAACCGCCACCGCCCGCCGACA CGCAGCCCCCGGGTGACGATGGCGGCCACGGGATAGGCGAAGAAGACCGCGAAGAAGGCGAGCGGCACCG CCATCAGGGCGAGCCGGAGGGCGATCGCGCGACGGCCGCGGTGGTCCGCCGTGTCGGCCTTACGGTGGCC GTCGGCCTTCTTACGGTTCTCGCGGGGCTCTACAGGACCAGCGAGGACCAT

Protein sequence (NCBI) or Translate to protein
Restriction digest
BLAST
Design primers with primer3

Gene(s) or part of gene(s) amplified:
ORF.. 4050840-4052570	Sequence	M271_15650 - iron ABC transporter permease (Protein is coded in complementary strand; from 4052570 to 4050840)
ORF... 4052549-4053622	Sequence	M271_15655 - ABC transporter substrate-binding protein (Protein is coded in complementary strand; from 4053622 to 4052549)